Clonal propagation and the fast generation of karyotype diversity: anin vitro Leishmaniamodel
| Autor: | S. De Doncker, D. Jacquet, Jean-Claude Dujardin, D. Le Ray, D. Van Bockstaele, M. Balavoine, Anne-Laure Bañuls, Michel Tibayrenc, Jorge Arevalo |
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| Rok vydání: | 2006 |
| Předmět: |
Clone (cell biology)
selection Aneuploidy Chromosomal size variation Protozoology Biology Clones Models Biological Evolutionary genetics Chromosomes Leishmania braziliensis Ploidy Culture Techniques Homologous chromosome medicine Animals Polymorphism pseudo sexuality Mitosis Leishmania peruviana Leishmania Genetics Life Cycle Stages Ploidies hybrid ploidy Chromosome Karyotype medicine.disease biology.organism_classification Clone Cells karvotype Infectious Diseases Karyotyping Animal Science and Zoology Parasitology Karyotypes Leishmania Viannia peruviana Genome Protozoan |
| Zdroj: | Parasitology |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182006001156 |
| Popis: | In the present work we studied the karyotype stability during long-termin vitromaintenance in 3 cloned strains ofLeishmania (Viannia) peruviana,Leishmania (Viannia) braziliensisand a hybrid between both species. Only theL. (V.) peruvianastrain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns duringin vitromaintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. The resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. No change in total ploidy of the cells was observed by flow cytometry. We discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. Our results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology. |
| Databáze: | OpenAIRE |
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