Therapeutic potential of combined anti-IL-1β IgY and anti-TNF-α IgY in guinea pigs with allergic rhinitis induced by ovalbumin
| Autor: | Zhou Wen-yun, He Dan, Hu Wei-xu, Wu Xiao-mu, Hu Guo-zhu, Wen Zhu, Zhu Xi-ling, Wu Li-hua |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Ovalbumin Guinea Pigs Interleukin-1beta Immunology Anti-Inflammatory Agents Immunoglobulin E Fluticasone propionate Allergic inflammation medicine Animals Humans Immunology and Allergy Antibodies Blocking Interleukin 5 Pharmacology biology Tumor Necrosis Factor-alpha business.industry Allergens Rhinitis Allergic Disease Models Animal Interleukin 13 biology.protein Drug Therapy Combination Nasal administration Immunotherapy Antibody business medicine.drug |
| Zdroj: | International Immunopharmacology. 25:155-161 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2014.12.002 |
| Popis: | We have previously demonstrated that anti-IL-1β immunoglobulin yolk(IgY) inhibits pathological responses in allergic asthma guinea pigs induced by ovalbumin(OVA). This study aims to determine whether the combined blockade of IL-1β and TNF-α can more effectively inhibit allergic inflammation in allergic rhinitis(AR) guinea pigs induced by OVA. Healthy guinea pigs treated with saline were used as the healthy control. The AR guinea pigs induced by OVA were randomly divided into (1) the AR model group containing negative control animals treated with intranasal saline; (2) the 0.1% non-specific IgY treatment group treated with non-specific IgY; (3) the 0.1% anti-TNF-α IgY treatment group treated with 0.1% anti-TNF-α IgY; (4) the 0.1% anti-IL-1β IgY treatment group treated with 0.1% anti-IL-1β IgY; (5) the 0.1% combined anti-IL-1β IgY and anti-TNF-α IgY treatment group treated with 0.1% combined anti-IL-1β IgY and anti-TNF-α IgY; and (6) the fluticasone propionate treatment group treated with fluticasone propionate. Cytokines were measured using an enzyme-linked immunosorbent assay. The results showed that IL-1β, IL-5, IL-9, IL-13, IL-18, IL-22, IL-33, TNF-α, TGF-β1 and OVA-specific IgE levels in the peripheral blood (PB) and nasal lavage fluid (NLF) significantly decreased at 2h, 4h or 8h in the 0.1% combined anti-IL-1β IgY and anti-TNF-α IgY treatment group compared to the AR model group and the 0.1% non-specific IgY treatment group (P |
| Databáze: | OpenAIRE |
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