Enhancing chondrogenic phenotype for cartilage tissue engineering: monoculture and coculture of articular chondrocytes and mesenchymal stem cells
| Autor: | Kelsea M. Hubka, Fred K Kasper, Rebecca L. Dahlin, Ville V. Meretoja, Antonios G. Mikos |
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| Rok vydání: | 2014 |
| Předmět: |
Cartilage
Articular Biomedical Engineering Bioengineering Biology Biochemistry Chondrocyte Biomaterials Chondrocytes medicine Animals Humans Review Articles Stem cell transplantation for articular cartilage repair Tissue Engineering Cartilage Regeneration (biology) Mesenchymal stem cell Mesenchymal Stem Cells Chondrogenesis Phenotype Coculture Techniques Cell biology medicine.anatomical_structure Fibrocartilage Biomedical engineering |
| Zdroj: | Tissue engineering. Part B, Reviews. 20(6) |
| ISSN: | 1937-3376 |
| Popis: | Articular cartilage exhibits an inherently low rate of regeneration. Consequently, damage to articular cartilage often requires surgical intervention. However, existing treatments generally result in the formation of fibrocartilage tissue, which is inferior to native articular cartilage. As a result, cartilage engineering strategies seek to repair or replace damaged cartilage with an engineered tissue that restores full functionality to the impaired joint. These strategies often involve the use of chondrocytes, yet in vitro expansion and culture can lead to undesirable changes in chondrocyte phenotype. This review focuses on the use of articular chondrocytes and mesenchymal stem cells (MSCs) in either monoculture or coculture for the enhancement of chondrogenesis. Coculture strategies increasingly outperform their monoculture counterparts with regard to chondrogenesis and present unique opportunities to attain chondrocyte phenotype stability in vitro. Methods to prevent chondrocyte dedifferentiation and promote chondrocyte redifferentiation as well as to promote the chondrogenic differentiation of MSCs while preventing MSC hypertrophy are discussed. |
| Databáze: | OpenAIRE |
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