A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone
| Autor: | Yael Helman, O. Schwartz-Harari, R. Nir-Paz, Talia Yarnitzky, Igor Kviatkovski, Sagit Shushan |
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| Rok vydání: | 2018 |
| Předmět: |
Lung Diseases
0301 basic medicine Luminescence Biosensing Techniques medicine.disease_cause Catalysis Microbiology 03 medical and health sciences Limit of Detection Materials Chemistry medicine Humans Aliivibrio fischeri Receptor Respiratory Tract Infections chemistry.chemical_classification Detection limit Binding Sites biology Pseudomonas aeruginosa Sputum Metals and Alloys Acetophenones Quorum Sensing General Chemistry biochemical phenomena metabolism and nutrition biology.organism_classification Surfaces Coatings and Films Electronic Optical and Magnetic Materials Amino acid Genetically modified organism Molecular Docking Simulation Repressor Proteins Biomarker Quorum sensing 030104 developmental biology chemistry Mutation Trans-Activators Ceramics and Composites bacteria Biomarkers |
| Zdroj: | Chemical Communications. 54:9218-9221 |
| ISSN: | 1364-548X 1359-7345 |
| Popis: | 2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections. |
| Databáze: | OpenAIRE |
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