Combined effects of high hydrostatic pressure and dispersed oil on the metabolism and the mortality of turbot hepatocytes (Scophthalmus maximus)
| Autor: | Stéphane Le Floch, Alexandra Marziou, Karine Pichavant-Rafini, Philippe Lemaire, Michael Theron, Matthieu Dussauze |
|---|---|
| Přispěvatelé: | Optimisation des régulations physiologiques (ORPHY (EA 4324)), Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
Health Toxicology and Mutagenesis 0208 environmental biotechnology Hydrostatic pressure chemistry.chemical_element Context (language use) 02 engineering and technology 010501 environmental sciences Ecotoxicology 01 natural sciences Oxygen Dispersant Hydrostatic Pressure Animals Environmental Chemistry Petroleum Pollution 14. Life underwater Viability assay Food science Ecosystem 0105 earth and related environmental sciences biology Public Health Environmental and Occupational Health General Medicine General Chemistry Metabolism biology.organism_classification Pollution 020801 environmental engineering Scophthalmus Turbot Petroleum Seafood chemistry [SDE]Environmental Sciences Flatfishes Hepatocytes Water Pollutants Chemical |
| Zdroj: | Chemosphere Chemosphere, Elsevier, 2020, 249, pp.126420-. ⟨10.1016/j.chemosphere.2020.126420⟩ |
| ISSN: | 0045-6535 |
| Popis: | Since the DeepWater Horizon oil spill and the use at 1450 m depth of dispersant as a technical response, the need of relevant ecotoxicological data on deep-sea ecosystems becomes crucial. In this context, this study focused on the effect of high hydrostatic pressure (10.1 MPa) on turbot hepatocytes isolated from fish exposed either to chemically dispersed oil, mechanically dispersed oil or dispersant alone. Potential combined effects of oil/dispersant and hydrostatic pressure, were assessed on cell mortality (total cell death, necrosis and apoptosis), cell viability and on hepatocyte oxygen consumption (MO2). No change in cell mortality was observed in any of the experimental conditions, whereas, the results of cell viability showed a strong and significant increase in the two oil groups independently of the pressure exposure. Finally, oil exposure and hydrostatic pressure have additive effects on oxygen consumption at a cellular level. Presence of dispersant prevent any MO2 increase in our experimental conditions. These mechanistic effects leading to this increased energetic demand and its eventual inhibition by dispersant must be investigated in further experiments. |
| Databáze: | OpenAIRE |
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