Infection of bone marrow cells by dengue virus in vivo
| Autor: | Francois Villinger, Hui-Mien Hsiao, Kristina B. Clark, Sansanee Noisakran, Aftab A. Ansari, Nattawat Onlamoon, Guey Chuen Perng |
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| Rok vydání: | 2011 |
| Předmět: |
Blood Platelets
Cancer Research viruses CD34 Bone Marrow Cells Dengue virus Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Giant Cells Article Flow cytometry Immunophenotyping Dengue Plasma Antigen Antigens CD Chlorocebus aethiops Genetics medicine Animals Cell Lineage Viremia Molecular Biology Vero Cells medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction virus diseases Cell Biology Hematology Cell sorting Dengue Virus Viral Load Virology Molecular biology Macaca mulatta Coculture Techniques Reverse transcription polymerase chain reaction Microscopy Electron medicine.anatomical_structure Vero cell RNA Viral Bone marrow Megakaryocytes |
| Zdroj: | Experimental hematology. 40(3) |
| ISSN: | 1873-2399 |
| Popis: | Abnormal bone marrow (BM) suppression is one of the hallmarks of dengue virus (DENV) infection in patients. Although the etiology remains unclear, direct viral targeting of the BM has been reasoned to be a contributing factor. The present studies were carried out in an effort to determine the potential effect of DENV infection on the cellularity of BM using a previously established nonhuman primate model of DENV-induced coagulopathy. BM aspirates were collected at various times from the infected nonhuman primate and cells were phenotypically defined and isolated using standard flow cytometry (fluorescence-activated cell sorting). These isolated cells were subjected to detection of DENV utilizing quantitative real-time reverse transcription polymerase chain reaction, electron microscopy, and immunostaining techniques. DENV RNA was detectable by quantitative real-time reverse transcription polymerase chain reaction in BM specimens and the presence of DENV-like particles within platelet was confirmed by electron microscopy. Enumeration of BM cells revealed a transient surge in cellularity at day 1, followed by a gradual decline from days 2 to 10 post infection. Detailed phenotypic studies showed similar kinetics in the frequencies of CD41(+)CD61(+) cells, regardless of CD34 and CD45 expression. The CD61(+) cells were not only the predominant cells that stained for DENV antigen but fluorescence-activated cell sorting-assisted isolation of CD61(+) cells from the BM were shown to contain infectious DENV by coculture with Vero cells. These data support the view that intravenous infection of nonhuman primate with DENV leads to direct infection of the BM, which is likely to be a contributing factor for transient cell suppression in the peripheral blood characteristic of acute DENV infection. |
| Databáze: | OpenAIRE |
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