CD49f protein expression varies among genetic subgroups of B lymphoblastic leukemia and is distinctly low in KMT2A ‐rearranged cases
| Autor: | Laila Mnayer, Katrina Collins, Jolene L. Cardinali, Joseph A. DiGiuseppe |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology Integrin alpha6 Protein expression Pathology and Forensic Medicine Flow cytometry 03 medical and health sciences 0302 clinical medicine Antigen Precursor B-Cell Lymphoblastic Leukemia-Lymphoma hemic and lymphatic diseases medicine Humans biology medicine.diagnostic_test Lymphoblast Histone-Lysine N-Methyltransferase Cell Biology Molecular biology Minimal residual disease 030104 developmental biology KMT2A 030220 oncology & carcinogenesis biology.protein Hypodiploidy Hyperdiploidy Myeloid-Lymphoid Leukemia Protein |
| Zdroj: | Cytometry Part B: Clinical Cytometry. 100:243-248 |
| ISSN: | 1552-4957 1552-4949 |
| DOI: | 10.1002/cyto.b.21865 |
| Popis: | Background CD49f (integrin α6) is a useful marker for minimal residual disease (MRD) detection in B lymphoblastic leukemia and has recently been suggested to mediate infiltration of the central nervous system by leukemic B lymphoblasts. However, data regarding expression of CD49f protein in B lymphoblastic leukemia are limited, and whether CD49f protein expression varies among genetic subgroups of B lymphoblastic leukemia is unknown. Methods CD49f protein expression was characterized by flow cytometry in a series of 40 cases of B lymphoblastic leukemia, which included the genetic subgroups: KMT2A-rerranged, BCR-ABL1+, ETV6-RUNX1+, hypodiploidy, and hyperdiploidy. Results Expression of CD49f differed significantly among the five genetic subgroups studied, whether assessed by percentage of blasts positive for the antigen (p = .0001, Kruskal-Wallis) or median fluorescence intensity (MFI) (p = .0001, Kruskal-Wallis). Moreover, the percentage of CD49f+ blasts and MFI of CD49f were significantly lower in KMT2A-rearranged cases than in cases without KMT2A rearrangement (p = .0002 for both, Mann-Whitney). Conclusions CD49f protein expression varies among genetic subgroups of B lymphoblastic leukemia, and is distinctly low in KMT2A-rearranged cases. |
| Databáze: | OpenAIRE |
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