The Identification and Location of Succinyl Residues and the Characterization of the Interior Arabinan Region Allow for a Model of the Complete Primary Structure of Mycobacterium tuberculosis Mycolyl Arabinogalactan
| Autor: | Kay-Hooi Khoo, Michael S. Scherman, Jessica E. Prenni, Christopher D. Rithner, Suresh Bhamidi, Michael R. McNeil, Delphi Chatterjee |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy Molecular Structure biology Chemistry Molecular Sequence Data Succinic Acid Protein primary structure Glycobiology and Extracellular Matrices Mycobacterium tuberculosis Cell Biology biology.organism_classification Polysaccharide Galactans Models Biological Biochemistry Cell wall Polysaccharides Arabinogalactan Solubilization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Acid-fast Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 283:12992-13000 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m800222200 |
| Popis: | The complex cell wall of Mycobacterium tuberculosis is the hallmark of acid fast bacteria and is responsible for much of its physiological characteristics. Hence, much effort has been made to determine its primary structure. Such studies have been hampered by its extreme complexity. Also, its insolubility leads to difficulties determining the presence or absence of base labile groups. We have used an endogenous arabinase to solubilize the arabinan region of the cell wall and have shown using mass spectrometry and NMR that succinyl esters are present on O2 of the inner-branched 1,3,5-α-d-arabinofuranosyl residues. In addition, an inner arabinan region of 14 linear α-1,5 arabinofuranosyl residues has been identified. These and earlier results now allow the presentation of a model of the entire primary structure of the mycobacterial mycolyl arabinogalactan highlighted by three arabinan chains of 31 residues each. |
| Databáze: | OpenAIRE |
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