Isolation and characterization of bioactive xanthones from Hippocratea africana (Willd.)Loes.ex Engl. (Celastraceae)
| Autor: | Emmanuel E. Essien, Olorunfemi A. Eseyin, Marinella De Leo, Kola’ K. Ajibesin, Uwemedimo F. Umoh, Paul S. Thomas, Guido Flamini, Jude E. Okokon |
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| Rok vydání: | 2020 |
| Předmět: |
Male
antioxidant Antioxidant medicine.drug_class Hippocratea medicine.medical_treatment Xanthones Analgesic Ethyl acetate Anti-Inflammatory Agents Pain Secondary Metabolism Norathyriol Plant Roots Anti-inflammatory Antioxidants Celastraceae 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Drug Discovery medicine Animals Edema Ionization mass spectrometry anti-inflammatory 030304 developmental biology Pharmacology Inflammation 0303 health sciences Analgesics Traditional medicine biology Plant Extracts analgesic biology.organism_classification chemistry 030220 oncology & carcinogenesis Female Hippocratea africana xanthones Hippocrateaceae |
| Zdroj: | Journal of ethnopharmacology. 280 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Hippocratea africana root is used in African folk medicine for the treatment of several ailments, including pain and inflammation. Aim of the study To isolate anti-inflammatory and analgesic compounds from the roots of H. africana, with accompanying antioxidant potentials. Materials and methods Dichloromethane, ethyl acetate, and aqueous fractions of H. africana roots, and isolated compounds from the bioactive ethyl acetate fraction were evaluated for anti-inflammatory and analgesic activities using the xylene induced oedema in mice and thermal induced pain models, respectively. The antioxidant potentials of isolated compounds were tested in 2,2-diphenyl-1-picryhydrazyl radical and ferric reducing antioxidant power assays. Structures were elucidated on the basis of spectroscopic analyses, including 1D and 2D NMR experiments, ionization mass spectrometry, and comparison with literature data. Results Isoathyriol (1,3,7-trihydroxy-6-methoxyxanthone) and norathyriol (1,3,6,7-tetrahydroxyxanthone) were isolated from the potent anti-inflammatory and analgesic ethyl acetate fraction of H. africana roots. Isoathyriol and norathyriol demonstrated good anti-inflammatory, analgesic, and antioxidant properties compared with the standards used in each assay. Conclusions This study substantiates the use of H. africana root extract in the alleviation of inflammation and pain, and reports the characterization of secondary metabolites in H. africana and for the first time the presence of xanthones in Hippocratea genus. |
| Databáze: | OpenAIRE |
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