Human T-cell epitopes of the latex allergen Hev b 5 in health care workers
| Autor: | Jennifer Rolland, Cenk Suphioglu, Jay E. Slater, Susan C. McLellan, Michael Fraser Sutherland, Harini D De Silva, Robyn E O'Hehir |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Allergy Latex Hypersensitivity Latex Health Personnel T-Lymphocytes Immunology Epitopes T-Lymphocyte medicine.disease_cause Immunoglobulin E Epitope Interferon-gamma Allergen Antigen medicine Immunology and Allergy Humans Aged Plant Proteins biology business.industry Allergens Antigens Plant Middle Aged medicine.disease Virology Epitope mapping Latex allergy biology.protein Female Interleukin-5 business Epitope Mapping |
| Zdroj: | The Journal of allergy and clinical immunology. 105(5) |
| ISSN: | 0091-6749 |
| Popis: | Background: Latex allergy affects health care workers as a high-risk cohort. Hev b 5 is a major latex allergen reacting with serum IgE from 92% of latex-allergic health care workers. Because CD4 + T-cell recognition is central to the specific immune response to allergens, identification of dominant T-cell epitopes is important for the development of specific immunotherapy for latex allergy. Objective: Our purpose was to map T-cell epitopes of Hev b 5 in health care workers. Methods: Six latex-allergic health care workers (grade 3 to 4 enzyme allergosorbent test score) were studied. Peripheral blood latex specific 3-week T-cell lines were generated and screened for proliferative response to overlapping 20-mer peptides of Hev b 5. Supernatants collected at 48 hours were analyzed by ELISA for IL-5 and IFN-γ. Results: Dot immunoblotting with use of recombinant Hev b 5/maltose-binding protein indicated serum-specific IgE in 5 of 6 patients. T-cell reactivity to one or more Hev b 5 peptides was identified in these 5 donors, but not in the sixth. Hev b 5 (46-65) induced T-cell proliferation in all 5 donors. Hev b 5 (109-128) stimulated T cells from 3 of these patients. Proliferative responses were accompanied by substantial IL-5 secretion and minimal IFN-γ, indicating a T H 2-predominant cytokine profile. Conclusions: Five of 6 latex-allergic patients demonstrated T-cell responsiveness to Hev b 5 consistent with a major T-cell reactive latex allergen. Two T-cell immunodominant regions of Hev b 5 were identified, and reactivity to these sites was associated with strong IL-5 but minimal IFN-γ production. (J Allergy Clin Immunol 2000;105:1017-24.) |
| Databáze: | OpenAIRE |
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