Immobilizing proteins on the surface of yeast cells
| Autor: | Frans M. Klis, A. T. A. Mooren, H. Y. Toschka, M. P. Schreuder, C. T. Verrips |
|---|---|
| Přispěvatelé: | SILS (FNWI) |
| Rok vydání: | 1996 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Recombinant Fusion Proteins Saccharomyces cerevisiae Heterologous Administration Oral Gene Expression Bioengineering Peptide Antibodies Cell wall Glucan chemistry.chemical_classification Vaccines biology Cell Membrane Membrane Proteins biology.organism_classification Enzymes Immobilized Yeast Biodegradation Environmental Mannose-Binding Lectins chemistry Biochemistry Eukaryote Glycoprotein Mating Factor Peptides Biotechnology |
| Zdroj: | Trends in Biotechnology, 14, 115-120. Elsevier |
| ISSN: | 0167-7799 |
| Popis: | Yeast has a rigid cell wall comprising an outer layer of glycoproteins and an internal skeletal layer of glucan; heterologous proteins can be targeted to the glycoprotein layer and become covalently linked to the glucan skeleton. Yeast is a eukaryote that has 'generally regarded as safe' (GRAS) status, and is easy to cultivate, so it seems ideally suited for applications including the manufacture of recyclable, immobilized, biocatalysts, whole-cell vaccines, the presentation of peptide or antibody libraries, and the presentation of adhesion or metal-binding proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect