Serological response to pgp3 protein in animal and human chlamydial infections
| Autor: | Simone Magnino, Manuela Donati, Roberto Cevenini, Karine Laroucau, Letizia Ceglie, Costanza Mazzeo, Maria Renzi, Raffaella Baldelli, Antonietta Di Francesco, Elisa Storni |
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| Přispěvatelé: | Donati M., Laroucau K., Storni E., Mazzeo C., Magnino S., Di Francesco A., Baldelli R., Ceglie L., Renzi M., Cevenini R. |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
CHLAMYDIAL INFECTIONS
Blotting Western Enzyme-Linked Immunosorbent Assay Biology Microbiology law.invention Serology Plasmid Blood serum Bacterial Proteins law medicine Animals Humans Chlamydia Chlamydophila Antigens Bacterial General Veterinary SEROLOGY General Medicine Chlamydia Infections medicine.disease biology.organism_classification Virology Antibodies Bacterial Humoral immunity Recombinant DNA biology.protein PGP3 PROTEIN Antibody PLASMID |
| Popis: | Specific antibodies to plasmid-encoded protein pgp3 are known to be encountered in human Chlamydia (C.) trachomatis infections. In order to verify whether antibodies to this protein could be developed in animals infected with plasmid-carrying chlamydial strains, 454 animal sera were examined using a home-made pgp3 protein ELISA and Western blots (WB) of recombinant pgp3 protein from Chlamydophila (Cp.) psittaci. Likewise, 50 human sera were tested by ELISA and WB of recombinant pgp3 from C. trachomatis. The reactivity against pgp3 protein was compared to the reactivity against chlamydial elementary bodies (EBs) detected by microimmunofluorescence (MIF) test. The presence of pgp3-specific antibodies was demonstrated in most ducks and pigeons with Cp. psittaci infection detected by MIF, as well as in the majority of symptomatic cats and pigs infected with Cp. felis and C. suis, respectively, which reacted at high titres to Cp. felis and C. suis EBs by MIF. Moreover, most of the sera collected from patients with C. trachomatis culture-confirmed infection and seropositive to C. trachomatis by MIF, presented antibodies specific to C. trachomatis pgp3 recombinant protein. Therefore, pgp3 protein could be a useful marker of chlamydial infections in animals, as well as in humans. |
| Databáze: | OpenAIRE |
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