Recombinant canine adenovirus type-2 expressing TgROP16 provides partial protection against acute Toxoplasma gondii infection in mice
| Autor: | Xiu-Xiang Zhang, Xu Zhang, Kenneth Yongabi Anchang, Feng Weili, Auwalu Yusuf Abdullahi, Lin Lv, Xiao-Hu Wang, Shoujun Li, Zi-Guo Yuan, Xiu-Zhen Li, Chunxia Lu, Liqing Tu, Xia Lijun |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Protozoan Vaccines
0301 basic medicine Microbiology (medical) 030231 tropical medicine Protozoan Proteins Antibodies Protozoan Adenoviruses Canine Biology Recombinant virus Microbiology Madin Darby Canine Kidney Cells law.invention Mice 03 medical and health sciences Dogs 0302 clinical medicine Immune system Western blot law parasitic diseases Vaccines DNA Genetics medicine Animals Cytotoxic T cell Molecular Biology Ecology Evolution Behavior and Systematics Mice Inbred BALB C medicine.diagnostic_test Toxoplasma gondii Protein-Tyrosine Kinases biology.organism_classification Virology Vaccination Toxoplasmosis Animal 030104 developmental biology Infectious Diseases Recombinant DNA Cytokines Female Toxoplasma Spleen CD8 |
| Zdroj: | Infection, Genetics and Evolution. 45:447-453 |
| ISSN: | 1567-1348 |
| Popis: | We previously demonstrated that the survival time of BALB/c mice challenged with Toxoplasma gondii RH strain was prolonged by immunising the mice with a eukaryotic vector expressing the protein ROP16 of T. gondii. Building upon previous findings, we are exploring improved vaccination strategies to enhance protection. In this work, a novel recombinant canine adenovirus type 2 expressing ROP16 (CAV-2-ROP16) of T. gondii was constructed and identified to express ROP16 in Madin-Darby canine kidney cells (MDCK) cells by western blot (WB) and indirect immunofluorescence (IFA) assays. Intramuscular immunisation of BALB/c mice with CAV-2-ROP16 was performed to evaluate the humoral and cellular immune responses. This vaccination triggered significant humoral and cellular responses, including ROP16-stimulated lymphoproliferation (P0.05). Compared to control groups, the CAV-2-ROP16 immunised mice had high production of IFN-γ, IL-2 and IL-12 (P0.05), with a predominance of IgG2a production, but not IL-10 (P0.05), revealing that a predominant Th1-type response had developed. The cell-mediated cytotoxic activity with high levels of IFN-γ and TNF-α was significantly increased in both CD4 |
| Databáze: | OpenAIRE |
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