Recognition of Leuconostoc oenos strains by the use of DNA restriction profiles
| Autor: | M. Lamoureux, J.-F. Cavin, Charles Diviès, Hervé Prévost |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Genetics Base Sequence biology EcoRI DNA Restriction Enzymes RNA Probes General Medicine HindIII Applied Microbiology and Biotechnology Molecular biology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Restriction fragment RNA Bacterial genomic DNA Terminal restriction fragment length polymorphism Restriction enzyme RNA Ribosomal 16S biology.protein Amplified fragment length polymorphism Restriction fragment length polymorphism Leuconostoc Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 39:547-552 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/bf00205049 |
| Popis: | The chromosome of 41 Leuconostoc oenos strains obtained from collections in different countries was analysed with the aim of differentiating the strains. Pulsed field electrophoresis (TAFE) was used to separate large DNA fragments created by the restriction enzymes NotI, SfiI and ApaI, which specifically recognize guanines or cytosines. The genomic DNA of 11 strains was analysed initially with NotI and only four different restriction profiles were observed. The genome size ranged from 1.8 to 2.1 megabase pairs (Mbp). Constant field electrophoresis applied to DNA treatment with 19 different restriction enzymes showed that the size of the fragments obtained increased proportionally to the percentage G + C present at the site of restriction. EcoRI and HindIII profiles revealed that the zone between 9 and 23 kbp allowed differentiation of the strains tested. Thus, the 41 strains fell into 30 restriction groups using only two enzymes. Hybridization with a non-radioactive DNA probe coding for 16S rRNA revealed that there were two 16S genes on the chromosome. |
| Databáze: | OpenAIRE |
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