Fluidic Device System for Mechanical Processing and Filtering of Human Lipoaspirate Enhances Recovery of Mesenchymal Stem Cells
| Autor: | Jeremy A. Lombardo, Derek A. Banyard, Alan D. Widgerow, Jered B. Haun |
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| Rok vydání: | 2022 |
| Předmět: |
5.2 Cellular and gene therapies
Stem Cells Clinical Sciences Bioengineering Mesenchymal Stem Cells Regenerative Medicine Stem Cell Research Article Adipose Tissue Lipectomy Stem Cell Research - Nonembryonic - Human Adipocytes Humans Stem Cell Research - Nonembryonic - Non-Human Surgery Development of treatments and therapeutic interventions Metabolic and endocrine |
| Zdroj: | Plast Reconstr Surg Plastic and reconstructive surgery, vol 151, iss 1 |
| ISSN: | 0032-1052 |
| Popis: | BackgroundAdipose tissue is an easily accessible source of stem and progenitor cells that offers exciting promise as an injectable autologous therapeutic for regenerative applications. Mechanical processing is preferred over enzymatic digestion, and the most common method involves shuffling lipoaspirate between syringes and filtering to produce nanofat. Although nanofat has shown exciting clinical results, the authors hypothesized that new device designs could enhance recovery of stem/progenitor cells through optimization of fluid dynamics principles, integration, and automation.MethodsThe authors designed and fabricated the emulsification and micronization device (EMD) and the filtration device (FD) to replace the manual nanofat procedures. Using human lipoaspirate samples, the EMD and the FD were optimized and compared to traditional nanofat using ex vivo measurements of cell number, viability, and percentage of mesenchymal stem cells and endothelial progenitor cells.ResultsThe EMD produced results statistically similar to nanofat, and these findings were confirmed for a cohort of diabetic patients. Combining the FD with the EMD was superior to manually filtered nanofat in terms of both recovered cell percentages (>1.5-fold) and numbers (two- to three-fold). Differences were statistically significant for total mesenchymal stem cells and a DPP4 + /CD55 + subpopulation linked to improved wound healing in diabetes.ConclusionsThe new EMD and the FD improved mechanical processing of human lipoaspirate in terms of mesenchymal stem cell enrichment and number compared to traditional nanofat. Future work will seek to investigate the wound healing response both in vitro and in vivo, and to refine the technology for automated operation within clinical settings.Clinical relevance statementThe new devices improved mechanical processing of human lipoaspirate in terms of stem cell enrichment and number compared to traditional methods. Future work will seek to validate wound healing response and refine the technology for automated operation within clinical settings. |
| Databáze: | OpenAIRE |
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