Low cryoprotectant concentration rapid vitrification of mouse oocytes and embryos
| Autor: | Jie Liu, Gloria Y. Lee, Thomas L. Toth, Mehmet Toner, John D. Biggers |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cryoprotectant
General Biochemistry Genetics and Molecular Biology Andrology 03 medical and health sciences Mice 0302 clinical medicine Cryoprotective Agents medicine Animals Vitrification Blastocyst High concentration Cryoprotectant toxicity Cryopreservation 030219 obstetrics & reproductive medicine Zygote Chemistry 0402 animal and dairy science Reproducibility of Results Embryo 04 agricultural and veterinary sciences General Medicine Oocyte 040201 dairy & animal science medicine.anatomical_structure embryonic structures Oocytes General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 98 |
| ISSN: | 1090-2392 |
| Popis: | Vitrification of mammalian oocytes and embryos is typically a two-step procedure involving two solutions of increasing concentrations of cryoprotectants. In the present study, we report a simple vitrification protocol that uses low cryoprotectant concentration and a single medium (LCSM). This medium, along with the traditional high concentration two media (HCTM) protocol, was used to vitrify mouse oocytes, zygotes, and blastocysts using silica capillary, cryotop, cryolock, and 0.25 ml straws. Survival rates, two-cell rates, and blastocyst formation rates were compared for oocytes and zygotes vitrified using both protocols. Results show that the LCSM protocol was as good as or better than the traditional HCTM protocol for vitrifying mouse MII oocytes and zygotes using silica capillary, cryotop, and cryolock. On the other hand, for blastocysts, only silica capillary using LCSM had comparable results with the traditional HCTM protocol while cryolock and cryotop had significantly lower percentages of re-expanded and hatched blastocysts. Collapsing blastocysts prior to vitrification or longer duration for better cryoprotectant distribution in multicellular embryos may improve the outcome. In conclusion, the LCSM protocol, with one medium of much lower cryoprotectant concentrations and shorter equilibration time, reduces exposure to cryoprotectant toxicity while improves efficiency, consistency and reliability for mammalian oocyte and embryo preservation. |
| Databáze: | OpenAIRE |
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