Distribution of type-1 and type-2 angiotensin receptors in the normal human lung and in lungs from patients with chronic obstructive pulmonary disease
| Autor: | Johan Kips, Ra Pauwels, G Bilbe, Robert M. Carey, I Steyaert, M. de Gasparo, B De Paepe, Gillian R. Bullock, Helmy M. Siragy, M. Praet |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Angiotensin receptor Pathology medicine.medical_specialty Histology Pulmonary Fibrosis Biology Receptor Angiotensin Type 1 Pulmonary Disease Chronic Obstructive Antibody Specificity Forced Expiratory Volume Pulmonary fibrosis medicine Humans RNA Messenger Receptor Molecular Biology Lung In Situ Hybridization Insulin-like growth factor 1 receptor Aged Aged 80 and over Angiotensin II receptor type 1 Receptors Angiotensin Cell Biology respiratory system Middle Aged medicine.disease Angiotensin II Epithelium Medical Laboratory Technology medicine.anatomical_structure Female |
| Zdroj: | Histochemistry and cell biology. 115(2) |
| ISSN: | 0948-6143 |
| Popis: | This study was designed to examine the cellular distribution of the angiotensin II type-1 (AT1) and type-2 (AT2) receptors in the normal human and pathological human lung. Riboprobes were prepared against specific portions of each receptor DNA and labelled with FITC for detection using an anti-FITC antibody in combination with the alkaline phosphatase-anti-alkaline phosphatase technique and new Fuchsin. These were used to detect the presence of receptor mRNA in the lung. Specific antibodies were used to detect receptor protein in cells by immunocytochemistry. Image analysis was used in order to semi-quantify receptor density. AT1 receptor mRNA and protein were localised on vascular smooth muscle cells, macrophages and in the stroma underlying the airways epithelium probably relating to underlying fibroblasts. The AT1 receptor protein was not expressed in the epithelium although there was a low level of mRNA. In contrast, AT2 receptor RNA and protein was observed in the epithelium, with strong staining on the bronchial epithelial cell brush border and also on many of the underlying mucous glands. The AT2 receptor was also present on some endothelial cells. These findings were supported by the presence of mRNA in each case. In patients with chronic obstructive pulmonary disease, there was a five- to sixfold increase in the ratio of AT1 to AT2 receptors in the regions of marked fibrosis surrounding the bronchioles. This correlated well with the reduced lung function as expressed by the forced expiratory volume. |
| Databáze: | OpenAIRE |
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