Redox characterization of usnic acid and its cytotoxic effect on human neuron-like cells (SH-SY5Y)
| Autor: | José Cláudio Fonseca Moreira, José Luiz Rybarczyk-Filho, João Paulo Almeida dos Santos, Thallita Kelly Rabelo, Fares Zeidán-Chuliá, Matheus Augusto de Bittencourt Pasquali, Laura Milán Vasques, Adriano Antunes de Souza Araújo, Ricardo Fagundes da Rocha, Daniel Pens Gelain |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
SH-SY5Y
Cell Survival Free radicals Nitric Oxide Toxicology medicine.disease_cause Thiobarbituric Acid Reactive Substances SH-SY5Y cells Nitric oxide Superoxide dismutase chemistry.chemical_compound Cellular viability In vivo Cell Line Tumor medicine Humans Hydrogen peroxide Benzofurans biology Hydroxyl Radical Superoxide Dismutase Usnic acid Hydrogen Peroxide General Medicine In vitro chemistry Biochemistry Oxidative stress biology.protein Oxidation-Reduction |
| Zdroj: | Toxicology in Vitro. 26:304-314 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2011.12.003 |
| Popis: | Usnic acid (UA) is the most common and abundant lichenic secondary metabolite with potential therapeutic application. Anti-inflammatory and antitumour properties have already been reported and UA-enriched extracts are widely used to treat several diseases in the folk medicine. First, we performed in silico evaluation of UA interactions with genes/proteins and important compounds for cellular redox balance and NO pathway. Then, we assessed UA redox properties against different reactive species (RS) generated in vitro, and evaluated its action on SH-SY5Y neuronal like cells upon hydrogen peroxide (H(2)O(2)), since no in vitro neurotoxicological data has been reported so far. Total reactive antioxidant potential index (TRAP) showed a significant antioxidant capacity of UA at the highest tested concentration; UA was also effective against hydroxyl radicals and reduced the formation of nitric oxide. In vitro, lipoperoxidation was enhanced by UA and changed the cellular viability at highest concentration of 20μg/mL for 1 and 4h, as well as 2 and 20μg/mL for 24h of treatment, according to MTT reduction assay. Moreover, UA did not display protective effects against H(2)O(2)-induced cell death in any case. Evaluation of intracellular RS production by the DCFH-based assay indicated that UA was able to induce changes in basal RS production at concentration of 20μg/mL for 1h and from 2ng/mL to 20μg/mL for 4 and 24h. In conclusion, UA could display variable redox-active properties, according to different system conditions and/or cellular environment. Moreover, our results suggest that potential neurotoxicological effects of UA should be further studied by additional approaches; for instance, in vivo and clinical studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect