Residual SARS-CoV-2 viral antigens detected in GI and hepatic tissues from five recovered patients with COVID-19
| Autor: | Shihleone Loong, Benedict Tan, Jia Lin Ng, Jenny G. Low, Denise Goh, Ye Xin Koh, Chung Yip Chan, Kiat Hon Lim, Justina Nadia Lee, Peng Chung Cheow, Jeffrey Chun Tatt Lim, Joe Yeong, Chun Chau Lawrence Cheung, Tracy Zhijun Tien, Xinru Lim, Wei Yee Wan, Thuan Tong Tan, Zhi En Amos Tay, Shirin Kalimuddin, Wai Meng David Tai, Eileen Xueqin Chen, Sanjna Nilesh Nerurkar |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty viruses Ileum Biology 03 medical and health sciences 0302 clinical medicine Immune system medicine Humans Antigens Viral SARS-CoV-2 Gastroenterology COVID-19 RNA medicine.disease Negative stain Staining 030104 developmental biology medicine.anatomical_structure Liver Hepatocellular carcinoma RNA Viral Immunohistochemistry 030211 gastroenterology & hepatology Lymph |
| Zdroj: | Gut. 71:226-229 |
| ISSN: | 1468-3288 0017-5749 |
| DOI: | 10.1136/gutjnl-2021-324280 |
| Popis: | We read with great interest the article published by Zuo et al , which highlighted the presence of SARS-CoV-2 RNA in stool samples during active and convalescence phases of COVID-19 infection.1 However, no study has reported the presence of viral antigens within GI and hepatic organs during the convalescent phase. Using conventional immunohistochemistry, we detected SARS-CoV-2 nucleocapsid protein (NP) in the colon, appendix, ileum, haemorrhoid, liver, gallbladder and lymph nodes (figure 1A–K) from five patients who recovered from COVID-19, ranging from 9 to 180 days after testing negative for SARS-CoV-2 (online supplemental table 1). Notably, when multiple tissues were obtained from one patient (patients 1 and 4), all the tissues showed the presence of the viral antigen, suggesting widespread multiorgan involvement of the viral infection. Interestingly, for the colon, the viral antigen was only present in normal colonic crypts and polyps but not in the neoplastic tissues (figure 1Q). Similar negative staining in the hepatocellular carcinoma tumour region was also observed (figure 1R) albeit the positive staining in some of the scattered immune cells (figure 1D). Validating our findings, we detected SARS-CoV-2 spike protein (figure 1L–P) and RNA (figure 2B–F) in the above-mentioned tissues using conventional immunohistochemistry and RNAscope, respectively. However, we were unable to detect viral RNA in some patients’ tissues (online supplemental table 1), possibly because of higher RNA degradation rate as compared with protein and other patient-dependent factors such as disease severity, time since recovery and basal metabolic rate.### Supplementary data [gutjnl-2021-324280supp001.pdf] Figure 1 Immunohistochemical staining of the SARS-CoV-2 nucleocapsid and spike proteins in intestinal and hepatic tissues. (A and B) Positive SARS-CoV-2 nucleocapsid protein (NP) staining in colonic crypts (A) and appendix (B), both with a granular supranuclear cytoplasmic pattern. (C) positive SARS-CoV-2 NP staining in scattered immune cells … |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|