Photoinduced cutaneous inflammatory response by psoralens
| Autor: | Narendranath S. Ranadive, Janak R. Kumar, Herbert F. Haberman, I. Aravind Menon |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Light UVA Radiation Erythema Neutrophils Ultraviolet Rays Administration Topical Inflammatory response Biophysics Vascular permeability Pharmacology Reference Values medicine Animals Radiology Nuclear Medicine and imaging Irradiation Skin Inflammation Radiation Lagomorpha Dose-Response Relationship Drug Radiological and Ultrasound Technology biology Chemistry Darkness biology.organism_classification Regional Blood Flow Toxicity Ultraviolet irradiation Methoxsalen Rabbits medicine.symptom |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 14:125-137 |
| ISSN: | 1011-1344 |
| Popis: | Our studies describe the inflammatory response in rabbit skin induced by topical application of 8-methoxypsoralen (8-MOP) and UVA-visible irradiation (320-700 nm). Increase in vascular permeability (iVP) and accumulation of polymorphonuclear leucocytes (aPMN) at the test sites were quantitated using 125I-albumin and 51Cr-labelled PMNs respectively. Erythema was graded visually. 8-MOP cream was applied topically and irradiated. The erythemal response, aPMN and iVP at the test sites were quantitated at 6, 24, 48 and 72 h post-irradiation. The iVP and aPMN were maximal at 24 h; the erythemal response was the same at 24-48 h. The responses were dependent on 8-MOP concentration and irradiation dose. Topical application of 200 micrograms 8-MOP cream followed by irradiation for 2 h (9.4 J cm-2) produced 3-7 times iVP, 2-4 times aPMN and intense erythema at the test sites after 24 h. Neither aPMN nor iVP was detected before 6 h and erythemal response was not observable up to 16 h after irradiation. The aPMN and iVP gradually subsided in 72 h, although the erythemal response was still present. The repeated exposure of 8-MOP-treated sites for three consecutive days 24 h apart did not produce appreciable iVP or aPMN at 72 h or 24 h after the last exposure; however, erythema persisted. The 8-MOP-treated sites previously exposed for three consecutive days on reapplication of 8-MOP cream plus irradiation showed significantly less response compared with non-pretreated sites. Our results suggest that the erythemal response is not directly related to either iVP or aPMN. |
| Databáze: | OpenAIRE |
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