IQ67 DOMAIN proteins facilitate preprophase band formation and division-plane orientation
| Autor: | Pratibha Kumari, Sabine Müller, Malte Kölling, Pantelis Livanos, Steffen Abel, Arvid Hermann, Gina Stamm, Luise Zergiebel, Katharina Bürstenbinder, Pradeep Dahiya, Yvonne Poeschl |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cell division Green Fluorescent Proteins Arabidopsis Vesicular Transport Proteins Symmetric cell division Plant Science Phragmoplast Microtubules Prophase 01 natural sciences 03 medical and health sciences Protein Domains Gene Expression Regulation Plant Microtubule Plant Cells Sulfanilamides Tobacco Cell cortex Phylogeny Arabidopsis Proteins Chemistry Cell plate Plants Genetically Modified Cell biology Dinitrobenzenes 030104 developmental biology Mutation Preprophase band Cytokinesis 010606 plant biology & botany |
| Zdroj: | Nature Plants. 7:739-747 |
| ISSN: | 2055-0278 |
| DOI: | 10.1038/s41477-021-00923-z |
| Popis: | Spatiotemporal control of cell division is essential for the growth and development of multicellular organisms. In plant cells, proper cell plate insertion during cytokinesis relies on the premitotic establishment of the division plane at the cell cortex. Two plant-specific cytoskeleton arrays, the preprophase band (PPB) and the phragmoplast, play important roles in division-plane orientation and cell plate formation, respectively1. Microtubule organization and dynamics and their communication with membranes at the cortex and cell plate are coordinated by multiple, mostly distinct microtubule-associated proteins2. How division-plane selection and establishment are linked, however, is still unknown. Here, we report members of the Arabidopsis IQ67 DOMAIN (IQD) family3 as microtubule-targeted proteins that localize to the PPB and phragmoplast and additionally reside at the cell plate and a polarized cortical region including the cortical division zone (CDZ). IQDs physically interact with PHRAGMOPLAST ORIENTING KINESIN (POK) proteins4,5 and PLECKSTRIN HOMOLOGY GTPase ACTIVATING (PHGAP) proteins6, which are core components of the CDZ1. The loss of IQD function impairs PPB formation and affects CDZ recruitment of POKs and PHGAPs, resulting in division-plane positioning defects. We propose that IQDs act as cellular scaffolds that facilitate PPB formation and CDZ set-up during symmetric cell division. The IQ67 DOMAIN (IQD) family are plant-specific calmodulin-binding proteins. Several members are associated with the microtubule cytoskeleton, and now IQD6, IQD7 and IQD8 are characterized as functioning in the set-up of the cell division plane in the root meristem of Arabidopsis. |
| Databáze: | OpenAIRE |
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