Induction of apoptosis by fucoidan isolated from a traditional food, Saccharina longissima, in U937 human leukemia cells
Autor: | Takeshi Teruya, Mariko Nago, Junpei Shimabukuro, Takuya Yogi, Teruko Konishi, Masakuni Tako |
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Rok vydání: | 2018 |
Předmět: |
Population
Medicine (miscellaneous) lcsh:TX341-641 Biochemistry chemistry.chemical_compound fucoidan human leukemia cells medicine brown seaweed education traditional food lcsh:R5-920 education.field_of_study Nutrition and Dietetics U937 cell biology Chemistry Cell growth Fucoidan Monocyte apoptosis biology.organism_classification Molecular biology medicine.anatomical_structure Apoptosis Cell culture Saccharina Saccharine longissimi lcsh:Medicine (General) lcsh:Nutrition. Foods and food supply Food Science |
Zdroj: | Functional Foods in Health and Disease, Vol 8, Iss 4, Pp 242-255 (2018) |
ISSN: | 2160-3855 2378-7007 |
Popis: | Background : A brown seaweed, Saccharina longissima ( Laminaria angustata var. longissima ) has been consumed in Okinawa for 300 years. It is native to the southernmost part of Japan, in Okinawa, but grows on the Pacific coast (Kushiro and Nemuro) in Hokkaido in the northernmost part of Japan. Previously, we reported the structural characteristics from S. longissima . The fucoidan consisted of L-fucose, D-galactose, D-glucose, D-xylose, D-glucuronic acid, and sulfuric acid. This fucoidan activated the RAW 264.7 murine macrophage cell line. The aim of this study was to investigate the effects of a fucoidan isolated from Saccharina longissima on cell anti-proliferation and apoptosis in the U937 human leukemia monocyte lymphoma cell line. Methods : U937 cells were incubated with four fucoidan concentrations. The degree of apoptosis was determined using the APOPercentage APOPtosis assay, caspaer-3 activity assay and Western blotting analysis assay. Results : The treatment of cell with a fucoidan highly substituted with sulfate, in addition to L-fucose residues, inhibited cell growth. Apoptosis increased in U937 cells in a dose-dependent manner following the addition of fucoidan. However, the fucoidan did not induce apoptosis in the presence of caspase inhibitor (z-VAD-FMK). Conclusions : The fucoidan from Saccharina longissima induced apoptosis in U937 cells. Accordingly, the kelp containing this fucoidan may contribute to the observed longevity of the Okinawan population. Keywords: fucoidan, Saccharina longissimi, brown seaweed, traditional food, human leukemia cells, apoptosis |
Databáze: | OpenAIRE |
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