Synthesis and composition of glycosaminoglycans by cultured human trabecular meshwork cells
| Autor: | Johannes W. Rohen, D. O. Schachtschabel, J. Wever, Klaus Sames |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Aging Population Perlecan In Vitro Techniques Dermatan sulfate Cell Line Glycosaminoglycan Cellular and Molecular Neuroscience chemistry.chemical_compound Trabecular Meshwork Hyaluronic acid medicine Humans Chondroitin sulfate education Cells Cultured Glycosaminoglycans education.field_of_study biology Heparan sulfate Middle Aged Molecular biology Sensory Systems carbohydrates (lipids) Ophthalmology medicine.anatomical_structure chemistry biology.protein Trabecular meshwork |
| Zdroj: | Graefe's Archive for Clinical and Experimental Ophthalmology. 218:113-117 |
| ISSN: | 1435-702X 0721-832X |
| DOI: | 10.1007/bf02215647 |
| Popis: | A human trabecular meshwork cell line with a limited number of population doublings was established in monolayer culture. All cultures produced hyaluronic acid, heparan sulfate, chondroitin sulfate, and dermatan sulfate. Following [14C]-glucosamine incorporation into proliferating (phase II) cultures, 70%--80% of the medium glycosaminoglycan label was found in hyaluronic acid and 8%--14% in heparin sulfate. About 60% of the cell-bound activity was associated with hyaluronic acid and 30% with heparan sulfate. Long-term cultivation under nondividing ("senescent") conditions resulted in a change of the pattern of synthesized and excreted (medium) [14C]-glucosamine-labeled glycosaminoglycans with a relative decrease of hyaluronic acid and a relative increase of heparan sulfate. |
| Databáze: | OpenAIRE |
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