Polyclonal Antibody-Based Noncompetitive Immunoassay for Small Analytes Developed with Short Peptide Loops Isolated from Phage Libraries
| Autor: | A. González-Techera, H. J. Kim, S. J. Gee, J. A. Last, B. D. Hammock, G. González-Sapienza |
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| Rok vydání: | 2007 |
| Předmět: |
Phage display
medicine.drug_class Enzyme-Linked Immunosorbent Assay Peptide Monoclonal antibody Sensitivity and Specificity Antibodies Article Analytical Chemistry Bacteriophage Peptide Library medicine Bacteriophages Amino Acid Sequence Peptide library chemistry.chemical_classification medicine.diagnostic_test biology biology.organism_classification Molecular biology Biochemistry chemistry Polyclonal antibodies Immunoassay biology.protein Hapten |
| Zdroj: | Analytical Chemistry. 79:9191-9196 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac7016713 |
| Popis: | To date, there are a few technologies for the development of noncompetitive immunoassays for small molecules, the most common of which relies on the use of anti-immunocomplex antibodies. This approach is laborious, case specific, and relies upon monoclonal antibody technology for its implementation. We recently demonstrated that, in the case of monoclonal antibody-based immunoassays, short peptide loops isolated from phage display libraries can be used as substitutes of the anti-immunocomplex antibodies for noncompetitive immunodetection of small molecules. The aim of this work was to demonstrate that such phage ligands can be isolated even when the selector antibodies are polyclonal in nature. Using phenoxybenzoic acid (PBA), a major pyrethroid metabolite, as a model system, we isolated the CFNGKDWLYC peptide after panning a cyclic peptide library on the PBA/anti-PBA immunocomplex. The sensitivity of the noncompetitive enzyme-linked immunosorbent assay (ELISA) setup with this peptide was 5-fold (heterologous) or 400-fold (homologous) higher than that of the competitive assay setup with the same antibody. Phage anti-immunocomplex assay (PHAIA) was also easily adapted into a rapid and highly sensitive dipstick assay. The method not only provides a positive readout but also constitutes a major shortcut in the development of sensitive polyclonal-based assays, avoiding the need of synthesizing heterologous competing haptens. |
| Databáze: | OpenAIRE |
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