Angiopathic consequences of saturating the plasma scavenger system for actin
| Autor: | M Guoth, G G Pietra, Joseph W. Sanger, John G. Haddad, K D Harper |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Vitamin D-binding protein
Enzyme-Linked Immunosorbent Assay Plasma protein binding macromolecular substances Biology In vivo Calcium-binding protein Animals Humans Lung Actin Gelsolin Fluorescent Dyes Multidisciplinary Macrophages Muscles Myocardium Vitamin D-Binding Protein Calcium-Binding Proteins Microfilament Proteins Rats Inbred Strains Fluoresceins Blood proteins Molecular biology In vitro Actins Rats Microscopy Electron Biophysics Rabbits Fluorescein-5-isothiocyanate Thiocyanates Research Article Protein Binding |
| Popis: | Two plasma proteins, vitamin D-binding protein (actin monomer sequestrant) and gelsolin (actin polymer severing), have been found in association with actin in plasma from ill humans and during experimental injury. In vitro, these are the only plasma proteins that display a high affinity for actin. We infused increasing amounts of globular actin intravenously to rats to evaluate its disposition in plasma and tissues. Intravascular filament formation, microthrombi, and endothelial injury were observed, especially in the pulmonary circulation. These pathological changes were not observed when the globular actin in the infusate had been preincubated with the vitamin D-binding protein in vitro. Complexes of actin with both proteins were found in the plasma, suggesting a saturable, plasma actin-binding system in vivo. Our findings suggest that in vivo saturation of these proteins' actin-binding capacities may serve as a paradigm for pulmonary vascular disorders seen during widespread tissue trauma and cell lysis. |
| Databáze: | OpenAIRE |
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