Circulating Platelet-derived and Placenta-derived Microparticles Expose Flt-1 in Preeclampsia
| Autor: | Joris A. M. van der Post, Christine A. R. Lok, Augueste Sturk, Ian L. Sargent, Rienk Nieuwland, Suren R. Sooranna, Anita N. Böing |
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| Přispěvatelé: | ACS - Amsterdam Cardiovascular Sciences, CCA -Cancer Center Amsterdam, Other Research, Laboratory for Experimental Clinical Chemistry, ARD - Amsterdam Reproduction and Development, Obstetrics and Gynaecology, Laboratory for General Clinical Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Blood Platelets Placenta Preeclampsia Flow cytometry Andrology Pre-Eclampsia Western blot Cell-Derived Microparticles Pregnancy medicine Humans Platelet Vascular Endothelial Growth Factor Receptor-1 medicine.diagnostic_test business.industry Infant Newborn Obstetrics and Gynecology medicine.disease equipment and supplies Normotensive pregnancy medicine.anatomical_structure Immunology embryonic structures cardiovascular system Female sense organs Vascular function business circulatory and respiratory physiology |
| Zdroj: | Reproductive sciences (Thousand Oaks, Calif.), 15(10), 1002-1010. SAGE Publications Inc. |
| ISSN: | 1933-7205 |
| Popis: | BACKGROUND: Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing microparticles. METHODS: The concentration of Flt-1 was measured in samples from preeclamptic patients, pregnant and nonpregnant women by enzyme-linked immunosorbent assay. Microparticles were analyzed by flow cytometry. Western blot determined the different forms of Flt-1. RESULTS: Noncell bound Flt-1 was elevated in preeclampsia compared to controls. A fraction (5%) was associated with microparticles in preeclampsia. Flt-1-exposing microparticles were increased in preeclampsia compared to normotensive pregnancy (p = 0.02). Full-length Flt-1, was identified in microparticles of platelet and placental origin. CONCLUSION: Full-length Flt-1 is associated with platelet and placenta-derived microparticles. Possibly, the presentation of Flt-1 on the membrane of a microparticle might alter its function, particularly if it acts in synergism with other exposed vasoactive molecules. |
| Databáze: | OpenAIRE |
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