Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

Autor: Gabriela Egly Feresin, Carlos M Villegas Gabutti, Mario J. Simirgiotis, Beatriz Lima, Jésica Daniela Paredes, Alejandro Tapia, Graciela Haydée Wendel, Jorge Bórquez, Carlos Gamarra-Luques, Lorena Luna, Jessica Gómez, A. O. M. María
Rok vydání: 2019
Předmět:
Antioxidant
DPPH
medicine.medical_treatment
Pharmaceutical Science
Decoction
CINNAMIC ACIDS DERIVATIVES
01 natural sciences
Antioxidants
Mass Spectrometry
Analytical Chemistry
Lipid peroxidation
chemistry.chemical_compound
Phytomedicine
Drug Discovery
Chromatography
High Pressure Liquid
0303 health sciences
biology
Traditional medicine
Baccharis
cinnamic acids derivatives
Chemistry (miscellaneous)
Molecular Medicine
purl.org/becyt/ford/3 [https]
Antibacterial activity
Argentinean plants
purl.org/becyt/ford/3.5 [https]
Article
Cinnamic acid
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
Picrates
ARGENTINEAN PLANTS
medicine
Humans
UHPLC Orbitrap (Q-OT)
Physical and Theoretical Chemistry
030304 developmental biology
010405 organic chemistry
Biphenyl Compounds
Organic Chemistry
FLAVONOIDS
HCT116 Cells
biology.organism_classification
0104 chemical sciences
UHPLC ORBITRAP (Q-OT)
chemistry
Cinnamates
flavonoids
Lipid Peroxidation
Zdroj: Molecules
Volume 24
Issue 6
Molecules, Vol 24, Iss 6, p 1085 (2019)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1420-3049
Popis: The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µ
g/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µ
g/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.
Databáze: OpenAIRE
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