Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2
Autor: | Chalobol Wongsawad, Atcharaphan Wanlop, Pheravut Wongsawad, Pongphol Prattapong, Jong-Yil Chai, Thapana Chontananarth |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
030231 tropical medicine ITS2 Zoology Centrocestus formosanus Heterophyidae Carassius auratus Trematode Infections Brief Communication Host-Parasite Interactions Cyprinus Fish Diseases 03 medical and health sciences 0302 clinical medicine Databases Genetic parasitic diseases Ornamental plant Prevalence Sucker Animals Parasite hosting Metacercariae Genes Helminth Phylogeny biology ornamental fish 030108 mycology & parasitology Thailand biology.organism_classification Infectious Diseases Poecilia Sailfin molly Parasitology Chiang Mai |
Zdroj: | The Korean Journal of Parasitology |
ISSN: | 1738-0006 0023-4001 |
Popis: | The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed. |
Databáze: | OpenAIRE |
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