Systemic administration of transforming growth factor-beta 2 prevents the impaired bone formation and osteopenia induced by unloading in rats
| Autor: | Pierre J. Marie, M. Hott, Danielle Godet, Mohamed Machwate, Erik Zerath, Xavier Holy, Abderrahim Lomri |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Stromal cell Bone Marrow Cells Bone and Bones Bone Density Bone Marrow Reference Values Transforming Growth Factor beta Internal medicine medicine Animals Humans RNA Messenger Rats Wistar Cells Cultured Bone Development biology Chemistry Osteoblast General Medicine Transforming growth factor beta medicine.disease Recombinant Proteins Biomechanical Phenomena Rats Osteopenia Bone Diseases Metabolic Endocrinology medicine.anatomical_structure biology.protein Osteocalcin Systemic administration Alkaline phosphatase Collagen Cell Division Type I collagen Research Article |
| Zdroj: | Journal of Clinical Investigation. 96:1245-1253 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci118158 |
| Popis: | We investigated the effect of recombinant human transforming growth factor beta 2 (rhTGF-beta 2) administration on trabecular bone loss induced by unloading in rats. Hind limb suspension for 14 d inhibited bone formation and induced osteopenia as shown by decreased bone volume, calcium and protein contents in long bone metaphysis. Systemic infusion of rhTFG-beta 2 (2 micrograms/kg per day) maintained normal bone formation rate, and prevented the decrease in bone volume, bone mineral content, trabecular thickness and number induced by unloading. In vitro analysis of tibial marrow stromal cells showed that rhTGF-beta 2 infusion in unloaded rats increased the proliferation of osteoblast precursor cells, but did not affect alkaline phosphatase activity or osteocalcin production. Northern blot analysis of RNA extracted from the femoral metaphysis showed that rhTGF-beta 2 infusion in unloaded rats increased steady-state levels of type I collagen mRNA but not alkaline phosphatase mRNA levels. rhTGF-beta 2 infusion at the dose used had no effect on metaphyseal bone volume and formation, osteoblast proliferation or collagen expression in control rats. The results show that systemic administration of rhTGF-beta 2 enhances osteoblast precursor cell proliferation and type I collagen expression by osteoblasts, and prevents the impaired bone formation and osteopenia induced by unloading. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|