Rapid Screening of Lectins for Multivalency Effects with a Glycodendrimer Microarray
| Autor: | Rob M. J. Liskamp, Roland J. Pieters, Raymond Kooij, Marjolein van der Kaaden, Valentin Wittmann, Rob Ruijtenbeek, Núria Parera Pera, Hilbert M. Branderhorst, Caroline Maierhofer |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Dendrimers
Microarray Carbohydrates Biochemistry dendrimers Binding profile Lectins Dendrimer inhibitors Protein–carbohydrate interactions microarrays Molecular Biology biology Chemistry Ligand Organic Chemistry Lectin Microarray Analysis Spectrometry Fluorescence ddc:540 click chemistry biology.protein Click chemistry Molecular Medicine DNA microarray Protein Binding |
| Zdroj: | ChemBioChem. 11:1896-1904 |
| ISSN: | 1439-4227 |
| Popis: | Multivalency is an important phenomenon in protein–carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text