Establishment and Characterization of Immortalized Human Amniotic Epithelial Cells
| Autor: | Toshio Nikaido, Tohru Kiyono, Motonori Okabe, Kaixuan Zhou, Moustafa Fathy, Shigeru Saito, Toshiko Yoshida, Chika Koike, Satoru Kyo |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Homeobox protein NANOG
Cellular differentiation Papillomavirus E7 Proteins Biology Stem cell marker Kruppel-Like Factor 4 SOX2 Antigens CD Spheroids Cellular Humans Telomerase reverse transcriptase Amnion Telomerase Cell Line Transformed Amniotic stem cells Cell Differentiation Epithelial Cells Cell Biology Original Articles Molecular biology Amniotic epithelial cells embryonic structures Cancer research Stem cell Developmental Biology Biotechnology Transcription Factors |
| Popis: | Human amniotic epithelial cells (HAEs) have a low immunogenic profile and possess potent immunosuppressive properties. HAEs also have several characteristics similar to stem cells, and they are discarded after parturition. Thus, they could potentially be used in cell therapy with fewer ethical problems. HAEs have a short life, so our aim is to establish and characterize immortalized human amniotic epithelial cells (iHAEs). HAEs were introduced with viral oncogenes E6/E7 and with human telomerase reverse transcriptase (hTERT) to create iHAEs. These iHAEs have proliferated around 200 population doublings (PDs) for at least 12 months. High expression of stem cell markers (Oct 3/4, Nanog, Sox2, Klf4) and epithelial markers (CK5, CK18) were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). These iHAEs were expanded in ultra-low-attachment dishes to form spheroids similarly to epithelial stem/precursor cells. High expression of mesenchymal (CD44, CD73, CD90, CD105) and somatic (CD24, CD29, CD271, Nestin) stem cell markers was detected by flow cytometry. The iHAEs showed adipogenic, osteogenic, neuronal, and cardiac differentiation abilities. In conclusion, the immortalization of HAEs with the characteristics of stem cells has been established, allowing these iHAEs to become useful for cell therapy and regenerative medicine. |
| Databáze: | OpenAIRE |
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