Transcriptome Analysis to Identify Human Spermatogonial Cells from Sertoli Cell-Only Testes
| Autor: | Masanori Tabara, Koji Shiraishi, Hideyasu Matsuyama |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Urology Cell Separation DNA sequencing Transcriptome Sertoli cell-only syndrome 03 medical and health sciences 0302 clinical medicine Testis Medicine Humans RNA Messenger RNA-Seq Spermatogenesis Gene Azoospermia 030219 obstetrics & reproductive medicine Sertoli Cells business.industry Middle Aged medicine.disease Sertoli cell Spermatogonia Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis business Biomarkers |
| Zdroj: | The Journal of urology. 203(4) |
| ISSN: | 1527-3792 |
| Popis: | We sought to determine whether gene products from spermatogenic cells could be detected in Sertoli cell-only testes. Transcriptome analysis using next generation sequencing was performed using human testicular samples.This study enrolled 20 men with nonobstructive azoospermia with a histological diagnosis of Sertoli cell-only and no germ cells on in vitro fertilization analysis and 5 with obstructive azoospermia and histologically normal spermatogenesis. Individual differences in the transcriptome of obstructive azoospermia testicular tissues generated on the Illumina® platform were compared as the number of fragments per kilobase of exon per million mapped sequence reads. We confirmed mRNA expression and targeted gene product localization by quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical analysis, respectively.Using next generation sequencing we analyzed 5,666 of the 23,003 screened genes. As representative markers of immature germ cells,A number of Sertoli cell-only testes contain immature germ cells (spermatogonia and spermatogenic stem cells) with various gene expression profiles. Sertoli cell-only is a heterogeneous pathophysiology which may be corrected by medical treatment or regenerative technologies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|