Gel electrophoresis-autoradiographic image analysis of radiolabeled protein drug concentration in serum for pharmacokinetic studies
| Autor: | Soo Peang Khor, Di Song, Shang Ma |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Toxicology
Iodine Radioisotopes Rats Sprague-Dawley Pharmacokinetics Liquid chromatography–mass spectrometry Image Processing Computer-Assisted Animals Humans Polyacrylamide gel electrophoresis Pharmacology Gel electrophoresis Chromatography Chemistry Receptors Interleukin-13 Albumin Receptors Interleukin Interleukin-13 Receptor alpha1 Subunit Recombinant Proteins Bioavailability Rats Electrophoresis Autoradiographic Image Injections Intravenous Autoradiography Electrophoresis Polyacrylamide Gel Female Biotechnology |
| Zdroj: | Journal of pharmacological and toxicological methods. 47(1) |
| ISSN: | 1056-8719 |
| Popis: | Introduction: The purpose of this study was to evaluate the feasibility of using gel electrophoresis combined with autoradiographic image analysis for quantitating protein drug concentrations in biological fluid for pharmacokinetic studies. Methods: Protein drugs were iodinated using the Iodogen reagent and injected into Sprague–Dawley rats for pharmacokinetic evaluation. Serum samples were analyzed using trichloroacetic acid (TCA)-precipitable counts or sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Commercially available precasted Bis–Tris gradient gels were used for SDS-PAGE. Autoradiography of gel samples was performed using phosphoimager and quantitated using the ImageQuant software. Results: The maximum loading volume for protein drugs with molecular weight close to that of albumin (∼70 kDa) was about 1 μl, whereas for protein drugs with larger or smaller molecular weight (i.e., >80 or |
| Databáze: | OpenAIRE |
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