Human Epidermal Langerhans Cells Replenish Skin Xenografts and Are Depleted by Alloreactive T Cells In Vivo
| Autor: | Ralf G. Meyer, Rudolf E. Schopf, Matthias Theobald, Joanna Wegner-Kops, Maya C. André, Wolfgang Herr, Diana Wolff, Julia Hemmerling, Udo F. Hartwig, Esther von Stebut, Eva Wagner |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Pathology medicine.medical_specialty T cell Cellular differentiation Transplantation Heterologous Immunology Graft vs Host Disease Mice Transgenic Human skin Mice SCID CD8-Positive T-Lymphocytes Biology Lymphocyte Activation Mice Cell Movement Mice Inbred NOD In vivo medicine Animals Humans Immunology and Allergy Cells Cultured Cell Proliferation Mice Knockout Cell Death integumentary system Epidermis (botany) Cell Differentiation Skin Transplantation Dendritic cell Transplantation Disease Models Animal Haematopoiesis medicine.anatomical_structure Langerhans Cells Cancer research Female Epidermis |
| Zdroj: | The Journal of Immunology. 187:1142-1149 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1001491 |
| Popis: | Epidermal Langerhans cells (LC) are potent APCs surveying the skin. They are crucial regulators of T cell activation in the context of inflammatory skin disease and graft-versus-host disease (GVHD). In contrast to other dendritic cell subtypes, murine LC are able to reconstitute after local depletion without the need of peripheral blood-derived precursors. In this study, we introduce an experimental model of human skin grafted to NOD-SCID IL2Rγnull mice. In this model, we demonstrate that xenografting leads to the transient loss of LC from the human skin grafts. Despite the lack of a human hematopoietic system, human LC repopulated the xenografts 6 to 9 wk after transplantation. By staining of LC with the proliferation marker Ki67, we show that one third of the replenishing LC exhibit proliferative activity in vivo. We further used the skin xenograft as an in vivo model for human GVHD. HLA-disparate third-party T cells stimulated with skin donor-derived dendritic cells were injected intravenously into NOD-SCID IL2Rγnull mice that had been transplanted with human skin. The application of alloreactive T cells led to erythema and was associated with histological signs of GVHD limited to the transplanted human skin. The inflammation also led to the depletion of LC from the epidermis. In summary, we provide evidence that human LC are able to repopulate the skin independent of blood-derived precursor cells and that this at least partly relates to their proliferative capacity. Our data also propose xeno-transplantation of human skin as a model system for studying the role of skin dendritic cells in the efferent arm of GVHD. |
| Databáze: | OpenAIRE |
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