Single nucleotide polymorphisms and serologic levels of hypoxia-inducible factor1 α and vascular endothelial growth factor are associated with increased risk of oral submucous fibrosis in gutka users among a North Indian population
| Autor: | Alpana Sharma, Kalaivani Mani, Shalini R. Gupta, Nidhi Gupta |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Tobacco Smokeless Angiogenesis Single-nucleotide polymorphism Oral Submucous Fibrosis Pilot Projects Gastroenterology Polymorphism Single Nucleotide Pathology and Forensic Medicine Malignant transformation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Carcinoma Humans Radiology Nuclear Medicine and imaging Dentistry (miscellaneous) Prospective Studies Prospective cohort study Hypoxia business.industry 030206 dentistry Odds ratio medicine.disease Hypoxia-Inducible Factor 1 alpha Subunit Vascular endothelial growth factor Oral submucous fibrosis chemistry 030220 oncology & carcinogenesis Carcinoma Squamous Cell Surgery Mouth Neoplasms Oral Surgery business |
| Zdroj: | Oral surgery, oral medicine, oral pathology and oral radiology. 130(5) |
| ISSN: | 2212-4411 |
| Popis: | Tissue hypoxia in oral submucous fibrosis (OSMF) induces hypoxia-inducible factor (HIF)-1 α and vascular endothelial growth factor (VEGF), causing angiogenesis. Single nucleotide polymorphisms (SNPs) may predict susceptibility to environmental carcinogens and to development of OSMF, as well as its severity and malignant transformation. This study aimed to determine the serologic levels and frequencies of SNPs of HIF-1 α and VEGF in OSMF.In this prospective pilot study, the frequencies of SNPs of HIF-1 α (C1772 T, G1790 A); VEGF-A 936 C/T; and VEGF-C (rs7664413, rs1485766) in patients with OSMF or oral squamous cell carcinoma (OSCC) and in healthy controls were determined by using polymerase chain reaction (PCR) (n = 100 each), and serologic levels were determined by using enzyme-linked immunosorbent assay (ELISA (n = 50 each), in a North Indian population.Heterozygous forms of HIF-1 α C1772 T (CT: odds ratio [OR] 5.0; 95% confidence interval [CI] 2.24-11.16; P.001); HIF-1 α G1790 A (GA: OR 2.8; 95% CI 1.62-5.16; P.001); and VEGF-C rs1485766 (AC: OR 2.18; 95% CI 1.19-3.99; P.05) were associated with OSMF. The mean serologic levels of HIF-1 α, VEGF-A, and VEGF-C were significantly raised in patients with OSMF compared with healthy controls (P.001).The SNPs of HIF-1 α, VEGF-A, and VEGF-C and their serologic levels can act as prognostic biomarkers and aid in the development of specialized anti-HIF-1 α or anti-VEGF drugs for the management and prevention of OSCC in patients with OSMF. |
| Databáze: | OpenAIRE |
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