A DNA damage-induced, SOS-independent checkpoint regulates cell division in Caulobacter crescentus
| Autor: | Barrett S. Perchuk, Joshua W. Modell, Tracy K. Kambara, Michael T. Laub |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biology, Modell, Joshua W., Kambara, Tracy K., Perchuk, Barrett, Laub, Michael T. |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cell cycle checkpoint
Cell division DNA damage QH301-705.5 genetic processes Mutation Missense Microbiology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Suppression Genetic Bacterial Proteins Caulobacter crescentus SOS response Biology (General) 030304 developmental biology 0303 health sciences General Immunology and Microbiology biology 030306 microbiology General Neuroscience DNA replication Biology and Life Sciences Membrane Proteins Bacteriology Cell Cycle Checkpoints biochemical phenomena metabolism and nutrition biology.organism_classification 3. Good health Cell biology Synopsis bacteria Repressor lexA General Agricultural and Biological Sciences Cytokinesis Cell Division Transcription Factors Research Article DNA Damage |
| Zdroj: | PLoS Biology, Vol 12, Iss 10, p e1001977 (2014) PLoS Biology Public Library of Science |
| ISSN: | 1545-7885 1544-9173 |
| Popis: | A study of the bacterium Caulobacter crescentus reveals an SOS-independent DNA damage response pathway that acts via a novel cell division inhibitor, DidA, to suppress septum synthesis. Cells must coordinate DNA replication with cell division, especially during episodes of DNA damage. The paradigm for cell division control following DNA damage in bacteria involves the SOS response where cleavage of the transcriptional repressor LexA induces a division inhibitor. However, in Caulobacter crescentus, cells lacking the primary SOS-regulated inhibitor, sidA, can often still delay division post-damage. Here we identify didA, a second cell division inhibitor that is induced by DNA damage, but in an SOS-independent manner. Together, DidA and SidA inhibit division, such that cells lacking both inhibitors divide prematurely following DNA damage, with lethal consequences. We show that DidA does not disrupt assembly of the division machinery and instead binds the essential division protein FtsN to block cytokinesis. Intriguingly, mutations in FtsW and FtsI, which drive the synthesis of septal cell wall material, can suppress the activity of both SidA and DidA, likely by causing the FtsW/I/N complex to hyperactively initiate cell division. Finally, we identify a transcription factor, DriD, that drives the SOS-independent transcription of didA following DNA damage. Author Summary Cells have evolved sophisticated mechanisms for repairing their DNA and maintaining genome integrity. A critical aspect of the repair process is an arrest of cell cycle progression, thereby ensuring that cell division is not attempted before the genome has been repaired and fully duplicated. Our paper explores the molecular mechanisms that underlie the inhibition of cell division following DNA damage in the bacterium Caulobacter crescentus. For most bacteria, the primary, and only mechanism previously described involves the SOS response, in which DNA damage induces cleavage of the transcriptional repressor LexA, driving induction of a battery of genes that includes an inhibitor of cell division (sulA in E. coli and sidA in Caulobacter). Here, we report that Caulobacter cells have a second, SOS-independent damage response pathway that induces another division inhibitor, didA, which works together with sidA to block cell division following DNA damage. We also identify the damage-sensitive transcription factor responsible for inducing DidA. Finally, our study demonstrates that DidA and SidA inhibit cell division in an atypical manner. Many division inhibitors in bacteria appear to inhibit the protein FtsZ, which forms a ring at the site of cell division. DidA and SidA, however, target a trio of proteins, FtsW/I/N, that help synthesize the new cell wall that will separate the daughter cells (the septum). In sum, our work expands our understanding of how bacterial cells respond to DNA damage and the mechanisms by which they regulate cell division. |
| Databáze: | OpenAIRE |
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