Genetic lineage labeling in zebrafish uncovers novel neural crest contributions to the head, including gill pillar cells
| Autor: | Ajeet Pratap Singh, Peter Konstantinidis, Christiane Nüsslein-Volhard, Mitchell P. Levesque, Alessandro Mongera, Yi Yen Chen |
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| Rok vydání: | 2013 |
| Předmět: |
Gills
Mesoderm animal structures SOX10 Biology Animals Genetically Modified Cranial neural crest medicine Animals Cell Lineage Craniate Molecular Biology Zebrafish DNA Primers Microscopy Confocal Integrases SOXE Transcription Factors Neural crest Embryonic Tissue Anatomy Zebrafish Proteins biology.organism_classification Biological Evolution Immunohistochemistry Cell biology Tamoxifen Skull medicine.anatomical_structure Neural Crest Head Cryoultramicrotomy Developmental Biology |
| Zdroj: | Development. 140:916-925 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | At the protochordate-vertebrate transition, a new predatory lifestyle and increased body size coincided with the appearance of a true head. Characteristic innovations of this head are a skull protecting and accommodating a centralized nervous system, a jaw for prey capture and gills as respiratory organs. The neural crest (NC) is a major ontogenetic source for the ‘new head’ of vertebrates and its contribution to the cranial skeleton has been intensively studied in different model organisms. However, the role of NC in the expansion of the respiratory surface of the gills has been neglected. Here, we use genetic lineage labeling to address the contribution of NC to specific head structures, in particular to the gills of adult zebrafish. We generated a sox10:ERT2-Cre line and labeled NC cells by inducing Cre/loxP recombination with tamoxifen at embryonic stages. In juvenile and adult fish, we identified numerous established NC derivatives and, in the cranium, we precisely defined the crest/mesoderm interface of the skull roof. We show the NC origin of the opercular bones and of multiple cell types contributing to the barbels, chemosensory organs located in the mouth region. In the gills, we observed labeled primary and secondary lamellae. Clonal analysis reveals that pillar cells, a craniate innovation that mechanically supports the filaments and forms gill-specific capillaries, have a NC origin. Our data point to a crucial role for the NC in enabling more efficient gas exchange, thus uncovering a novel, direct involvement of this embryonic tissue in the evolution of respiratory systems at the protochordate-vertebrate transition. |
| Databáze: | OpenAIRE |
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