A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with go proteins
| Autor: | I. Rutkowska-Wlodarczyk, Juan Lerma, Laurent Prézeau, J.-C. Bologna, María Isabel Aller, Sergio Valbuena |
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| Přispěvatelé: | European Commission, Ministerio de Ciencia e Innovación (España), Ministerio de Educación y Ciencia (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Proteomics Kainate receptor GTP-Binding Protein alpha Subunits Gi-Go Biology Neurotransmission Interactome Metabotropic Mice Receptors Kainic Acid Ganglia Spinal Animals Humans Protein Interaction Domains and Motifs Ion channel Mice Knockout Kainic Acid GluK1 General Neuroscience Kainate receptors Brain Articles In vitro Cell biology Protein Subunits HEK293 Cells Metabotropic receptor Go protein Female Noncanonical Protein Binding Ionotropic effect |
| Popis: | Kainate receptors (KARs) are found ubiquitously in the CNS and are present presynaptically and postsynaptically regulating synaptic transmission and excitability. Functional studies have proven that KARs act as ion channels as well as potentially activating G-proteins, thus indicating the existance of a dual signaling system for KARs. Nevertheless, it is not clear how these ion channels activate G-proteins and which of the KAR subunits is involved. Here we performed a proteomic analysis to define proteins that interact with the C-terminal domain of GluK1 and we identified a variety of proteins with many different functions, including a Go α subunit. These interactions were verified through distinct in vitro and in vivo assays, and the activation of the Go protein by GluK1 was validated in bioluminescence resonance energy transfer experiments, while the specificity of this association was confirmed in GluK1-deficient mice. These data reveal components of the KAR interactome, and they show that GluK1 and Go proteins are natural partners, accounting for the metabotropic effects of KARs. This work was supported by grants to J.L. from the Spanish Ministry of Science and Innovation (BFU2006-07138 and BFU2011-24084), CONSOLIDER (CSD2007-00023), and Prometeo/2011/086, and by the EU FP7 Capacities Project BIO-IMAGINE (Grant Agreement No. 264173). |
| Databáze: | OpenAIRE |
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