ICAM-1 dependent pathway is not involved in the development of neuronal apoptosis after transient focal cerebral ischemia
| Autor: | Sulpicio G. Soriano, Pieter Dikkes, Paul R. Hickey, Jose-Carlos Gutierrez-Ramos, Yanming F. Wang, Stuart A. Lipton |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Programmed cell death Neuropil Necrosis Ratón Ischemia Biotin Apoptosis DNA Fragmentation DNA laddering Biology Mice medicine Animals Molecular Biology Cerebral Cortex Mice Knockout Neurons ICAM-1 TUNEL assay Staining and Labeling General Neuroscience Intercellular Adhesion Molecule-1 medicine.disease Molecular biology Ischemic Attack Transient Immunology Neurology (clinical) medicine.symptom Deoxyuracil Nucleotides Developmental Biology |
| Zdroj: | Brain Research. 780:337-341 |
| ISSN: | 0006-8993 |
| Popis: | We examined brain sections from ICAM-1 deficient mice (−/−) and their nontransgenic littermates (+/+) after focal cerebral ischemia and reperfusion (I/R) for the presence of apoptosis. Despite the reduction in necrosis, the −/− mice had apoptotic cells in the ischemic hemisphere as shown by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) staining and DNA laddering. ICAM-1 deficiency minimizes necrosis but not apoptosis after temporary MCAO in mice, thereby leaving the potential for delayed neuronal cell death despite ICAM-1 inactivation. |
| Databáze: | OpenAIRE |
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