Structure and expression of Ri T-DNA from Agrobacterium rhizogenes in: Nicotiana tabacum
| Autor: | Richard Broglie, Mylene Durand-Tardif, David Tepfer, Jerry L. Slightom |
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| Přispěvatelé: | Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), Laboratoire de biologie de la rhizosphère, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1985 |
| Předmět: |
0106 biological sciences
STRUCTURE Agrobacterium Nicotiana tabacum 01 natural sciences 03 medical and health sciences Nucleic acid thermodynamics chemistry.chemical_compound Plasmid Structural Biology Transcription (biology) Gene expression [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences biology BIOLOGIE MOLECULAIRE biology.organism_classification Molecular biology Phenotype chemistry DNA 010606 plant biology & botany |
| Zdroj: | Journal of Molecular Biology Journal of Molecular Biology, Elsevier, 1985, 186, pp.557-564 |
| ISSN: | 0022-2836 1089-8638 |
| Popis: | The incorporation of transferred DNA (T-DNA) from the Ri plasmid of Agrobacterium rhizogenes into the chromosomal DNA of higher plants is correlated with the appearance of a complex phenotype. The transformed genotype and phenotype undergo Mendelian inheritance. Through studies of Ri T-DNA content and transcription in Nicotiana tabacum, we have delineated a particular part of this foreign DNA as the likely source of the transformed phenotype. One inducible/repressible aspect of the transformed phenotype is termed T' and is correlated with the presence of a supplementary Ri T-DNA-encoded transcript. This transcript is found specifically in leaves, whereas most of the other T-DNA transcripts are more abundant in roots. The T' phenotype does not appear to be due to structural changes in the Ri T-DNA. It is inherited in a dominant Mendelian fashion. We propose that the T' phenotype is caused by heritable changes in the regulation of Ri T-DNA expression. We comment on the potential of this system as a model for studying eukaryotic gene expression. |
| Databáze: | OpenAIRE |
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