Behavior of Endogenous Tumor-Associated Macrophages Assessed In Vivo Using a Functionalized Nanoparticle
| Autor: | Jose-Luiz Figueiredo, Matthias Nahrendorf, Andita Newton, Ralph Weissleder, Martin Etzrodt, Virna Cortez-Retamozo, Natalie Elpek, Antoine Leimgruber, Cedric Berger, Mikael J. Pittet, Filip K. Swirski, Thorsten R. Mempel, Peter Waterman, Rainer H. Kohler |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Diagnostic Imaging
Cancer Research Pathology medicine.medical_specialty Confocal Metal Nanoparticles Biology lcsh:RC254-282 03 medical and health sciences Mice 0302 clinical medicine In vivo Neoplasms medicine Macrophage Animals Research Articles 030304 developmental biology 0303 health sciences Tumor microenvironment Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction Macrophages Cancer medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immunohistochemistry Magnetic Resonance Imaging 3. Good health Tumor progression 030220 oncology & carcinogenesis Cancer research Adenocarcinoma Intravital microscopy |
| Zdroj: | Neoplasia: An International Journal for Oncology Research, Vol 11, Iss 5, Pp 459-468 (2009) |
| ISSN: | 1522-8002 1476-5586 |
| Popis: | Tumor-associated macrophages (TAMs) invade the tumor stroma in many cancers, yet their role is incompletely understood. To visualize and better understand these critical cells in tumor progression, we screened a portfolio of rationally selected, injectable agents to image endogenous TAMs ubiquitously in three different cancer models (colon carcinoma, lung adenocarcinoma, and soft tissue sarcoma). AMTA680, a functionally derivatized magneto-fluorescent nanoparticle, labeled a subset of myeloid cells with an “M2” macrophage phenotype, whereas other neighboring cells, including tumor cells and a variety of other leukocytes, remained unlabeled. We further show that AMTA680-labeled endogenous TAMs are not altered and can be tracked noninvasively at different resolutions and using various imaging modalities, e.g., fluorescence molecular tomography, magnetic resonance imaging, and multiphoton and confocal intravital microscopy. Quantitative assessment of TAM distribution and activity in vivo identified that these cells cluster in delimited foci within tumors, show relatively low motility, and extend cytoplasmic protrusions for prolonged physical interactions with neighboring tumor cells. Noninvasive imaging can also be used to monitor TAM-depleting regimen quantitatively. Thus, AMTA680 or related cell-targeting agents represent appropriate injectable vehicles for in vivo analysis of the tumor microenvironment. |
| Databáze: | OpenAIRE |
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