Anti‑angiogenic effect of Livistona?chinensis seed extract in?vitro and in?vivo
| Autor: | Wei Lin, Liangpu Zheng, Qunchuan Zhuang, Jinyan Zhao, Zhenfeng Hong, Zhiyun Cao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Tube formation Cancer Research biology Angiogenesis Articles biology.organism_classification Molecular biology Umbilical vein Vascular endothelial growth factor 03 medical and health sciences Chorioallantoic membrane chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Oncology chemistry In vivo 030220 oncology & carcinogenesis embryonic structures cardiovascular system Livistona chinensis MTT assay |
| Zdroj: | Oncology Letters. |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2017.7075 |
| Popis: | The present study aimed to detect the impact of the ethanol extract of the Livistona chinensis seed (EELC) on angiogenesis in human umbilical vein endothelial cells (HUVECs). A chorioallantoic membrane (CAM) assay was used to detect the anti-angiogenic activity of EELC in vivo. In vitro, the effect of EELC on the proliferation, migration and angiogenesis of HUVECs was determined by an MTT assay, a wound healing assay and a tube formation assay, respectively. The vascular endothelial growth factor (VEGF)-A and VEGF receptor (VEGFR)-2 protein and mRNA level were measured with ELISA and reverse transcription-semi-quantitative polymerase chain reaction. It was observed that EELC significantly decreased the formation of new vessels in the CAM assay. EELC inhibited the proliferation and migration of HUVECs. The extent of tube formation by HUVECs was also reduced by EELC. In addition, EELC treatment reduced the level of VEGF-A and VEGFR-2 mRNA and protein. The results suggest that EELC inhibits tumor angiogenesis through inhibiting the proliferation and migration of HUVECs, and by downregulating VEGF and VEGFR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|