Antibacterial effects of quaternary bis-phosphonium and ammonium salts of pyridoxine on Staphylococcus aureus cells: A single base hitting two distinct targets?
| Autor: | Mikhail V. Pugachev, Airat R. Kayumov, Elena V. Nikitina, Yurii G. Shtyrlin, Marina I. Zeldi, Sergey V. Sapozhnikov, Mikhail I. Bogachev, Vladimir E. Evtygin, Svetlana V. Kuznetsova, Nikita V. Shtyrlin |
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| Rok vydání: | 2015 |
| Předmět: |
Staphylococcus aureus
Physiology Phosphonium salt Bacillus subtilis Microbial Sensitivity Tests 010402 general chemistry medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology chemistry.chemical_compound Organophosphorus Compounds Staphylococcus epidermidis Drug Resistance Bacterial medicine Ammonium Phosphonium Microscopy Confocal biology 010405 organic chemistry Pyridoxine General Medicine Staphylococcal Infections biology.organism_classification 0104 chemical sciences Anti-Bacterial Agents Quaternary Ammonium Compounds Biochemistry chemistry Microscopy Fluorescence Microscopy Electron Scanning Antibacterial activity Biotechnology medicine.drug |
| Zdroj: | World journal of microbiologybiotechnology. 32(1) |
| ISSN: | 1573-0972 |
| Popis: | We studied the effects of quaternary bis-phosphonium and bis-ammonium salts of pyridoxine with lipophilic substituents on the survival and morphology of Staphylococcus aureus cells. We found that, while originating from the same base, they exhibit considerably different antimicrobial mechanisms. In the presence of Ca(2+) ions the MIC and MBC values of ammonium salt increased 100-fold, suggesting that Ca(2+) ions can successfully impede the membrane Ca(2+) ions exchange required for ammonium salt incorporation. In contrast, in the presence of quaternary phosphonium salt, the artificial capsular-like material was formed around the cells and the filamentous and chain-like growth of the cells was observed suggesting the disruption of the cell division mechanisms. Altogether, both pyridoxine derivatives successfully inhibited the growth of gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis) and Escherichia coli considerably, while demonstrated nearly no effect against Klebsiella pneumoniae and Pseudomonas aeruginosa. We suggest that due to their effects on distinct and likely complementary targets the derivatives of pyridoxine represent potentially perspective antibacterials with complicated adaptation and thus with lower risk of drug resistance development. |
| Databáze: | OpenAIRE |
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