Species-independent stimulation of osteogenic differentiation induced by osteoclasts
| Autor: | Yang Zhang, Marjolein Polman, Abdullah Faqeer Mohammad, Inge Hermens, Zhumei Zhuang, Huanan Wang, Jeroen JJP. van den Beucken |
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| Rok vydání: | 2022 |
| Předmět: |
Osteoblasts
Biophysics Osteoclasts Cell Differentiation Cell Biology Biochemistry Rats Mice Anabolic Agents All institutes and research themes of the Radboud University Medical Center Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Osteogenesis Animals Bone Resorption Molecular Biology |
| Zdroj: | Biochemical and Biophysical Research Communications, 606, pp. 149-155 Biochemical and Biophysical Research Communications, 606, 149-155 |
| ISSN: | 0006-291X |
| Popis: | The coupling of bone resorption and bone formation is well-recognized in the bone remodeling process, in which osteoblasts and osteoclasts are key players. However, the anabolic effect of human primary osteoclasts has rarely been reported as mouse and cell line derived osteoclasts were mostly used in previous reports. Therefore, a comprehensive comparison of mouse and human osteoclasts and their corresponding functions is needed to study cell-cell interactions between osteoclasts and osteoblasts. Osteoclasts from mouse and human origin were generated, characterized and compared, after which their anabolic effects on the osteogenic differentiation of mouse and human MSCs were assessed. Both murine RAW264.7 derived osteoclasts (mOCs) and primary human osteoclasts (hOCs) derived from buffy coats characteristically displayed multinuclearity, marked integrin β3 expression and enhanced TRAP activity. Despite comparable cell size, mOCs showed higher osteoclast density (number of osteoclasts per cm |
| Databáze: | OpenAIRE |
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