Cancer stem cell sorting from colorectal cancer cell lines by sedimentation field flow fractionation
| Autor: | Muriel Mathonnet, Marie-Odile Jauberteau, Serge Battu, Hussein Akil, Carole Mélin, Philippe J.P. Cardot, Aurélie Perraud |
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| Přispěvatelé: | Homéostasie Cellulaire et Pathologies (HCP), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Service d'Immunologie et immunogénétique [CHU Limoges], CHU Limoges, Université de Limoges (UNILIM), Service de Chirurgie digestive, endocrinienne et générale [CHU Limoges] |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Fetal Proteins
MESH: Cell Line Tumor Colorectal cancer Cell Adhesion Molecules Neuronal MESH: Antigens Neoplasm [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Antigens CD44 01 natural sciences Analytical Chemistry Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cancer stem cell Antigens CD Antigens Neoplasm Cell Line Tumor medicine Humans MESH: Antigens CD MESH: Cell Adhesion Molecules Neuronal MESH: Humans biology Chemistry Cell adhesion molecule MESH: Fetal Proteins 010401 analytical chemistry CD44 Epithelial cell adhesion molecule Cell sorting medicine.disease Epithelial Cell Adhesion Molecule MESH: Fractionation Field Flow Molecular biology MESH: Neoplastic Stem Cells Fractionation Field Flow 3. Good health 0104 chemical sciences Hyaluronan Receptors Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Neoplastic Stem Cells MESH: Cell Adhesion Molecules Cell Adhesion Molecules |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2012, 84 (3), pp.1549-56. ⟨10.1021/ac202797z⟩ |
| ISSN: | 0003-2700 1520-6882 |
| DOI: | 10.1021/ac202797z⟩ |
| Popis: | International audience; Recently, cancer stem cells (CSCs) have been identified in many types of cancers, such as colorectal cancer (CRC). CSCs seem to be involved in initiation, growth, and tumor metastasis, as well as in radio- and chemotherapy failures. CSCs appears as new biological targets for cancer therapy, requiring the development of noninvasive cell sorting methods. In this study, we used sedimentation field flow fractionation (SdFFF) to prepare enriched populations of CSCs from eight cell lines corresponding to different CRC grades. On the basis of phenotypic and functional characterizations, "hyperlayer" elution resulted in a fraction overexpressing CSC markers (CD44, CD166, EpCAM) for all cell lines. CSCs were eluted in the last fraction for seven out of eight cell lines, but in the first for HCT116. These results suggest, according to the literature, that two different pools of CSCs exist, quiescent and activated, which can both be sorted by SdFFF. Moreover, according to CSC properties, enriched fractions are able to form colonies. |
| Databáze: | OpenAIRE |
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