Extremely selective 'turn-on' fluorescence detection of hypochlorite confirmed by proof-of-principle neurological studies via esterase action in living cells
| Autor: | Hyunjeong Liew, Olga G. Tsay, Taehong Jun, Dhiraj P. Murale, David G. Churchill, Yoo-Hun Suh, Atul P. Singh |
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| Rok vydání: | 2013 |
| Předmět: |
Boron Compounds
Cell Survival Hypochlorite Biochemistry Esterase Benzoates Fluorescence Analytical Chemistry chemistry.chemical_compound Neuroblastoma Electrochemistry Tumor Cells Cultured Environmental Chemistry Humans Neutral ph Spectroscopy Fluorescent Dyes Molecular Structure Benzenesulfonates Esterases Glutathione Hypochlorous Acid chemistry Microscopy Fluorescence Cell culture Biophysics BODIPY Reactive Oxygen Species Oxidation-Reduction |
| Zdroj: | The Analyst. 138(10) |
| ISSN: | 1364-5528 |
| Popis: | Highly specific and sensitive fluorescence detection of hypochlorite in nonbiotic pure water (rapid "turn-on", ~400 fold, λ(em) ~ 560 nm) as well as in living neuronal cell cultures (neutral pH) involves oxidation of a 2-sulfide-2-benzoic acid pendent group in a new meso-thienyl-BODIPY donor-acceptor probe. |
| Databáze: | OpenAIRE |
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