Single-cell immune repertoire sequencing of B and T cells in murine models of infection and autoimmunity
| Autor: | Danielle Shlesinger, Kai-Lin Hong, Ghazal Shammas, Nicolas Page, Ioana Sandu, Andreas Agrafiotis, Victor Kreiner, Nicolas Fonta, Ilena Vincenti, Ingrid Wagner, Margot Piccinno, Alexandre Mariotte, Bogna Klimek, Raphael Dizerens, Marcos Manero-Carranza, Raphael Kuhn, Roy Ehling, Lester Frei, Keywan Khodaverdi, Camilla Panetti, Nicole Joller, Annette Oxenius, Doron Merkler, Sai T. Reddy, Alexander Yermanos |
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| Přispěvatelé: | University of Zurich, Yermanos, Alexander |
| Rok vydání: | 2022 |
| Předmět: |
2403 Immunology
2716 Genetics (clinical) Immunology Receptors Antigen T-Cell Autoimmunity 610 Medicine & health CD8-Positive T-Lymphocytes Lymphocytic Choriomeningitis Mice Inbred C57BL Disease Models Animal Mice 1311 Genetics Genetics Animals Peptides 11493 Department of Quantitative Biomedicine Genetics (clinical) |
| Zdroj: | Genes and Immunity, 23 |
| ISSN: | 1476-5470 1466-4879 |
| Popis: | Adaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past and current immune responses. Recent advances in single-cell sequencing enable recovery of the complete adaptive immune receptor sequences in addition to transcriptional information. Here, we recovered transcriptome and immune repertoire information for polyclonal T follicular helper cells following lymphocytic choriomeningitis virus (LCMV) infection, CD8+ T cells with binding specificity restricted to two distinct LCMV peptides, and B and T cells isolated from the nervous system in the context of experimental autoimmune encephalomyelitis. We could relate clonal expansion, germline gene usage, and clonal convergence to cell phenotypes spanning activation, memory, naive, antibody secretion, T-cell inflation, and regulation. Together, this dataset provides a resource for immunologists that can be integrated with future single-cell immune repertoire and transcriptome sequencing datasets. Genes and Immunity, 23 ISSN:1466-4879 ISSN:1476-5470 |
| Databáze: | OpenAIRE |
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