Increase in serum and salivary neutrophil gelatinase-associated lipocalin levels with increased periodontal inflammation
| Autor: | Tan, Aykut, Gürbüz, Nilgün, Özbalci, Furkan İlker, Koşkan, Özgür, Yetkin Ay, Zuhal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Inflammation Lipocalin Systemic inflammation Generalized periodontitis Gastroenterology Pathogenesis 030207 dermatology & venereal diseases 03 medical and health sciences Gingivitis 0302 clinical medicine Lipocalin-2 Internal medicine medicine Humans Periodontitis General Dentistry Periodontal Diseases Innate immunity business.industry Interleukin RK1-715 030206 dentistry medicine.disease Dentistry Original Article Female medicine.symptom business |
| Zdroj: | Journal of Applied Oral Science, Volume: 28, Article number: e20200276, Published: 28 SEP 2020 Journal of Applied Oral Science v.28 2020 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP Journal of Applied Oral Science, Vol 28 (2020) Journal of Applied Oral Science |
| Popis: | Objective: This study aimed to determine serum and salivary levels of neutrophil gelatinase-associated lipocalin (NGAL) and evaluate NGAL correlation with key anti-interleukin 10 (IL-10) and pro-inflammatory (IL-1β) cytokines in different severities of periodontal diseases. We also calculated the systemic inflammation using the periodontal inflamed surface area (PISA) to evaluate its correlation with NGAL in the study groups. Methodology: Eighty systemically healthy and non-smoking individuals were separated into four groups of 20: clinically healthy (Group 1), gingivitis (Group 2), stage I generalized periodontitis (Group 3, Grade A), and stage III generalized periodontitis (Group 4, Grade A). Sociodemographic characteristics and periodontal parameters were recorded, and PISA was calculated. The serum and salivary levels of interleukin (IL)-1β, IL-10, and NGAL were determined using the enzyme-linked immunosorbent assay (ELISA). Results: We observed a significant increase in serum and salivary NGAL levels from healthy to periodontitis groups (p=0.000). Group 2 presented significantly higher serum and salivary IL-10 levels and salivary IL-1β levels than Group 3 (p=0.000). Serum and salivary parameters (IL-1β, IL-10, and NGAL levels) were strongly positively correlated to periodontal parameters and PISA values (p=0.000). Groups 2 and 3 showed overlapping PISA values. Conclusion: The overlapping PISA values found in Groups 2 and 3 suggest that gingivitis might progress to a systemic inflammatory burden somewhat comparable to stage I periodontitis. This finding is supported by the higher serum and salivary cytokines/mediators levels in the gingivitis group than in stage I periodontitis group. Serum and salivary NGAL levels increased proportionally to disease severity and PISA. NGAL seems to play a role in the pathogenesis of periodontal disease, within the limitation of our study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|