Determination of dipyridamole in pharmaceutical preparations using square wave voltammetry
| Autor: | Renata Alves de Toledo, Luiz Henrique Mazo, Marilza Castilho |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Detection limit
Reproducibility Chromatography medicine.diagnostic_test Chemistry Chemistry Pharmaceutical Coefficient of variation Clinical Biochemistry Analytical chemistry Pharmaceutical Science Dipyridamole Repeatability Analytical Chemistry Pharmaceutical Preparations Hanging mercury drop electrode Spectrophotometry Drug Discovery Electrochemistry medicine Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Voltammetry Spectroscopy |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 36:1113-1117 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2004.09.011 |
| Popis: | An analytical methodology using square wave voltammetry (SWV) at a hanging mercury drop electrode (HMDE) was developed for the quantitative determination of dipyridamole (DIP), a drug used for the treatment of several cardiovascular diseases, in pharmaceutical tablets and injections of Persantin in phosphate buffer (pH 3.0; 0.1M). After optimization of the parameters for SWV, analytical curves were obtained for application in the range of 1.28 x 10(-6)M to 7.02 x 10(-6)M. It was found a detection limit (DL) of 1.88 x 10(-8)M (9.50 ng/ml). The repeatability and the reproducibility of the method were determinated by successive measurements of DIP solutions on the range of the analytical curve with a coefficient variation of 0.97% (n=5) and 1.15%, respectively. The apparent recoveries were obtained by the IUPAC recommended procedure using the second reduction peak. Recoveries obtained by SWV were compared with the UV-vis spectrophotometric method. It was found that the determination of DIP in Persantin tablets gave a mean value of 75.6+/-0.4 mg (100.8%) and 68.9+/-0.3 mg (91.8%) for SWV and UV-vis spectrophotometry, respectively. In the case of injections, it was found 10.4+/-0.1 mg (103.4%) and 9.9+/-0.2 mg (99.9%) for SWV and UV-vis spectrophotometry. Both apparent recoveries for the two types of formulations are in good accordance with the declared value of 75 mg (tablets) and 10 mg (injections). |
| Databáze: | OpenAIRE |
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