The outcome of Leishmania major experimental infection in BALB/c mice can be modulated by exogenously delivered iron
| Autor: | Gen´eviève Milon, Georgia Konidou, Sylvia Bisti, Johan R. Boelaert, Ketty Soteriadou, Fatini Papageorgiou |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_treatment
Iron T-Lymphocytes Immunology Antibodies Protozoan Leishmaniasis Cutaneous Nitric Oxide Synthase Type II Spleen Lymphocyte Activation BALB/c Mice medicine Immunology and Allergy Animals Leishmania major RNA Messenger chemistry.chemical_classification Mice Inbred BALB C biology biology.organism_classification Deferoxamine Nitric oxide synthase Interleukin 10 Cytokine medicine.anatomical_structure chemistry Transferrin biology.protein Cytokines Female Nitric Oxide Synthase medicine.drug |
| Zdroj: | European journal of immunology. 30(12) |
| ISSN: | 0014-2980 |
| Popis: | We previously established that Leishmania promastigotes express a transferrin receptor and that iron chelators inhibit promastigote growth in vitro. Thus, we were interested in modulating the vertebrate host iron pool and to monitor whether such changes will affect the outcome of L. major infection in BALB / c mice, inoculated in the footpad with 106 stationary phase promastigotes. Treatment of mice with desferrioxamine resulted in a slight delay of the development of cutaneous lesions. In contrast and unexpectedly, systemic iron delivery, at early time points of parasite delivery, significantly limited footpad pathology. Accordingly, parasite loads at the site of parasite delivery, the draining lymph node, liver and spleen were significantly reduced in iron-loaded mice. Importantly, the "protective" effect of iron delivery correlated with the presence, at the site of inoculation, of lower levels of IL-4 and IL-10 transcripts while both IFN-gamma and inducible nitric oxide synthase transcripts were at higher levels. The presence of more type 1 cytokine transcripts was further supported by the increased levels of IgG2a in their sera. These data strongly suggest that susceptibility to L. major as assessed in the footpad model is modifiable by interventions that alter the iron status of the host at early time points of parasite delivery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text